盐霉素及mTOR信号通路对前列腺癌DU145干细胞的影响

发布时间：2018-06-20 09:02

本文选题：盐霉素 + 肿瘤干细胞　；参考：《实用医学杂志》2017年13期

【摘要】：目的探讨盐霉素对前列腺癌DU145干细胞的影响及其可能的机制,为盐霉素的临床应用提供理论依据。方法盐霉素处理DU145细胞,以ALDH为肿瘤干细胞标记物,用流式细胞仪检测处理后ALDH阳性的DU145干细胞的百分比。盐霉素处理DU145细胞后,用Western Blot法检测mTOR信号通路相关蛋白m-TOR、p-70s6k、p-p70s6、p-s6等的表达变化。盐霉素结合mTOR信号通路抑制剂雷帕霉素处理DU145细胞,利用流式细仪检测ALDH阳性的DU145干细胞百分比。结果盐霉素显著性抑制ALDH阳性的DU145干细胞(抑制率为77.78%),是紫杉醇的2倍(抑制率为38.64%)。盐霉素抑制mTOR信号通路相关分子m-TOR、p-70s6k、p-p70s6、p-s6等蛋白的表达,具有时间依赖性和剂量依赖性。盐霉素结合雷帕霉素能够抑制ALDH阳性的DU145干细胞比例(抑制率为77.95%)。结论盐霉素可能通过抑制mTOR通路信号来发挥抑制肿瘤干细胞的作用。
[Abstract]:Objective to investigate the effect of salinomycin on prostate cancer DU145 stem cells and its possible mechanism, and to provide theoretical basis for the clinical application of salinomycin. Methods DU145 cells were treated with salinomycin and ALDH was used as the marker of tumor stem cells. Flow cytometry was used to detect the percentage of ALDH-positive DU145 stem cells. After treated with salinomycin, the expression of mTOR signaling pathway related protein m-TORp-70s6kN, p-p70s6, p-s6 and so on was detected by Western Blot. DU145 cells were treated with salinomycin combined with rapamycin, an inhibitor of mTOR signaling pathway, and the percentage of ALDH positive DU145 stem cells was detected by flow cytometry. Results Salinomycin significantly inhibited ALDH-positive DU145 stem cells (inhibition rate was 77.78%, twice as much as paclitaxel) (inhibition rate was 38.64%). Salinomycin inhibited the expression of mTOR signaling pathway related molecules m-TOR-p-70s6knp-p70s6p-s6 in a time-and dose-dependent manner. Salinomycin combined with rapamycin could inhibit the percentage of ALDH-positive DU145 stem cells (inhibition rate was 77.95%). Conclusion Salinomycin may inhibit tumor stem cells by inhibiting the signal of mTOR pathway.
【作者单位】：南华大学附属第二医院;湖南省岳阳市第一人民医院泌尿外科;粤北人民医院医院泌尿外科;南华大学第二临床学院;
【基金】：国家自然科学基金资助(编号:81241090) 衡阳市科技计划项目资助(编号:2016KJ33) 南华大学“蒸湘学者”基金资助
【分类号】：R737.25

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