超声引导下细针穿刺注射大肠埃希菌建立大鼠慢性细菌性前列腺炎模型的探讨

发布时间：2018-06-24 10:53

本文选题：慢性细菌性前列腺炎 + 大鼠　；参考：《第三军医大学学报》2015年19期

【摘要】：目的超声引导下细针穿刺大鼠前列腺注射大肠埃希杆菌制作慢性细菌性前列腺炎模型。方法选取成年雄性SD大鼠36只,清洁级,体质量280~300 g,采用抽签法分为空白对照组、传统慢性细菌性前列腺炎模型(tradition chronic bacterial prostatitis model,TCBP)组和超声引导慢性细菌性前列腺炎模型(ultrasound chronic bacterial prostatitis model,UCBP)组,每组12只。4周后通过光学显微镜直接观察比较每组大鼠前列腺组织病理变化,并通过统计学方法比较炎症评分、大鼠死亡率及制作模型的手术时间。结果 TCBP组炎症评分(腺体形态改变、炎性细胞浸润和纤维组织增生)分别为(3.22±0.31)、(3.71±0.37)、(3.80±0.29),UCBP组炎症评分分别为(3.24±0.36)、(3.65±0.41)、(3.70±0.33),均显著高于空白对照组(0.20±0.07、0.23±0.04、0.28±0.06,P0.05),且UCBP组大鼠死亡率及手术时间均低于TCBP组(P0.05)。结论超声引导下细针穿刺大鼠前列腺注射大肠埃希菌可用于制作稳定的大鼠慢性细菌性前列腺炎模型,与传统的建模方式相比具有方便、快捷、大鼠死亡率低的优点。
[Abstract]:Objective to establish a model of chronic bacterial prostatitis by injection of Escherichia coli into the prostate of rats with fine needle puncture guided by ultrasound. Methods Thirty-six adult male Sprague-Dawley rats with clean grade and body mass of 280 ~ 300g were selected and divided into blank control group by drawing lots. The pathological changes of prostatic tissue of 12 rats in each group were observed by optical microscope after 4 weeks in the traditional chronic bacterial prostatitis model (tradition chronic bacterial prostatitis model (TCBP) group and ultrasound guided chronic bacterial prostatitis model (ultrasound chronic bacterial prostatitis model UCBP group. The inflammatory score, rat mortality and operation time of model were compared by statistical method. Results the inflammatory scores (glandular morphology, inflammatory cell infiltration and fibrous tissue proliferation) in TCBP group were (3.22 卤0.31), (3.71 卤0.37), (3.80 卤0.29) and (3.24 卤0.36), (3.65 卤0.41), (3.70 卤0.33) in UCBP group, which were significantly higher than those in control group (0.20 卤0.07 卤0.23 卤0.04 卤0.28 卤0.06), and the mortality and operation time in UCBP group were lower than those in TCBP group (P0.05). Conclusion Ultrasound-guided injection of Escherichia coli into the prostate of rats can be used to establish a stable model of chronic bacterial prostatitis in rats, which has the advantages of convenience, rapidity and low mortality compared with the traditional modeling method.
【作者单位】：第三军医大学新桥医院泌尿外一科;
【基金】：第三军医大学临床科研基金(2009XLC22)~~
【分类号】：R697.33;R-332

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