促红细胞生成素对急性肾损伤中干细胞因子及其受体表达的影响

发布时间：2018-06-24 17:07

本文选题：干细胞因子 + 急性肾损伤　；参考：《青岛大学》2014年硕士论文

【摘要】：背景：细胞因子在减轻肾脏缺血再灌注损伤中的作用逐渐受到重视,干细胞因子具有造血系统以外的器官保护作用。目的：探讨大鼠急性肾损伤模型中干细胞因子(stem cell factor SCF)及其受体c-Kit表达的变化和促红细胞生成素预处理对其表达的影响。方法：成年雄性Wistar大鼠34只,采用夹闭双侧肾蒂建立缺血再灌注模型,缺血45分钟后再灌注24小时,随机分为假手术组(n=10)、缺血再灌注组(17=12)和促红细胞生成素组(n=12),促红细胞生成素组于建立模型前2小时一次性尾静脉注射重组人促红细胞生成素5000U/kg。采用免疫组化及图像分析技术检测各组肾组织中SCF及其受体c-Kit的表达变化,自动生化仪测定血清肌酐和尿素氮水平,常规苏木精-伊红染色观察肾组织病理学改变并计算肾小管损伤积分。结果：(1)SCF及c-Kit在肾组织中的表达仅限于肾小管区域。与假手术组比较,SCF和c-Kit在缺血再灌注组和促红细胞生成素组的表达均明显增高(P0.05,P0.01),促红细胞生成素组SCF表达高于缺血再灌注组(P0.05),但缺血再灌注组和促红细胞生成素组c-Kit表达差异无显著性意义(P0.05)。(2)促红细胞生成素组血清肌酐与尿素氮水平明显低于缺血再灌注组(P0.05),但高于假手术组(P0.05)。(3)与假手术组比较,缺血再灌注组及促红细胞生成素组肾组织均出现不同程度的损伤,促红细胞生成素组肾组织损伤程度较缺血再灌注组明显减轻。结论：缺血再灌注导致急性肾损伤发生时SCF及c-Kit表达升高,而促红细胞生成素对急性肾损伤的保护作用可能与上调SCF表达有关。
[Abstract]:Background: the role of cytokines in alleviating renal ischemia-reperfusion injury has been paid more attention. Stem cell factor plays an organ protective role outside the hematopoietic system.
Objective: To investigate the changes in the expression of stem cell factor SCF and its receptor c-Kit in the rat model of acute renal injury and the effect of erythropoietin preconditioning on its expression.
Methods: 34 adult male Wistar rats were established by clamping bilateral renal pedicle for ischemia reperfusion model, and reperfusion for 24 hours after 45 minutes of ischemia. They were randomly divided into sham operation group (n=10), ischemia reperfusion group (17=12) and erythropoietin group (n=12), erythropoietin group was injected into a recombinant human intravenous infusion group at one time before the establishment of the model. Erythropoietin 5000U/kg. was used to detect the expression of SCF and its receptor c-Kit in renal tissues by immunohistochemistry and image analysis. The level of serum creatinine and urea nitrogen was measured by automatic biochemical analyzer. The pathological changes of renal tissue were observed by routine hematoxylin eosin staining and the integral of renal tubule injury was calculated.
Results: (1) the expression of SCF and c-Kit in renal tissue was limited to the renal tubule region. Compared with the sham group, the expression of SCF and c-Kit in the ischemia-reperfusion group and the erythropoietin group increased significantly (P0.05, P0.01). The expression of SCF in the erythropoietin group was higher than that of the ischemia reperfusion group (P0.05), but the ischemia reperfusion group and the erythropoiesis were produced. There was no significant difference in the expression of c-Kit in the vegetarian group (P0.05). (2) the serum creatinine and urea nitrogen level in the erythropoietin group was significantly lower than that of the ischemia reperfusion group (P0.05), but higher than the sham operation group (P0.05). (3) the renal tissue of the ischemia reperfusion group and the erythropoietin group had varying degrees of damage and erythropoietin growth compared with the sham operation group. The injury degree of kidney tissue in the treated group was significantly lower than that in the ischemia-reperfusion group.
Conclusion: the expression of SCF and c-Kit in acute renal injury is increased by ischemia-reperfusion, and the protective effect of erythropoietin on acute renal injury may be related to the up-regulation of SCF expression.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R692.5

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