C3a、C5a受体缺失减轻IgA肾病肾损伤

发布时间：2018-06-25 13:29

  本文选题：Ig + A肾病　； 参考：《中国免疫学杂志》2015年09期

【摘要】：目的:探讨C3a、C5a及其受体在Ig A肾病发病中作用及潜在机制。方法:6~8周清洁级雌性BALB/c小鼠28只,阴性对照组、野生型组、C3a受体敲除组、C5a受体敲除组各7只,采用有活性的仙台病毒滴鼻经鼻黏膜感染联合尾静脉注射建立小鼠Ig A肾病模型,测定24 h尿蛋白量、血清尿素氮、血清肌酐;留取肾组织标本,通过直接免疫荧光法检测肾组织Ig A、C3沉积;采用PAS染色光镜下观察肾组织病理改变;采用RT-q PCR法检测肾组织中TNF-α、TGF-β、IL-1β、IL-6、MCP-1mRNA相对表达量。结果:15周后野生型组、C3a受体敲除组、C5a受体敲除组(实验组)24 h尿蛋白量高于阴性对照组,野生型组高于C3a受体敲除组及C5a受体敲除组,差异具有统计学意义;血清尿素氮及血清肌酐差异无统计学意义;实验组小鼠可见肾脏组织病理改变,且野生型组重于C3a受体敲除组及C5a受体敲除组,阴性对照组肾脏组织病理则无明显异常。实验组小鼠肾组织TNF-α、TGF-β、IL-1β、IL-6、MCP-1 mRNA相对表达量高于阴性对照组,同时野生型组高于C5a受体敲除组及C3a受体敲除组,另外IL-1β、IL-6、MCP-1的mRNA相对表达量C5a受体敲除组高于C3a受体敲除组。结论:C3a及C5a受体缺失可减轻Ig A肾病肾损伤,并且C3a受体缺失作用更加显著。
[Abstract]:Objective: to investigate the role and potential mechanism of C _ 3a and C _ 5a receptor in the pathogenesis of IgA nephropathy. Methods 28 BALB / c mice, 28 female BALB / c mice, 8 weeks clean, negative control group and 7 wild type C3a receptor knockout group were used to establish the model of IgA nephropathy by nasal drip of active Sendai virus (Sendai virus) and tail vein injection. Urine protein content, serum urea nitrogen, serum creatinine were measured at 24 h, renal tissue samples were collected and the deposition of IgA C 3 in renal tissue was detected by direct immunofluorescence, and pathological changes of renal tissue were observed under light microscope with pas staining. RT-Q PCR was used to detect the relative expression of TNF- 伪 -TGF- 尾 -IL-1 尾 -IL-6 and MCP-1 mRNA in renal tissue. Results after 15 weeks, the urine protein content of the wild-type C3a receptor knockout group (experimental group) was higher than that of the negative control group, while the wild type group was higher than the C3a receptor knockout group and the C5a receptor knockout group. The difference was statistically significant. There was no significant difference in serum urea nitrogen and serum creatinine. The pathological changes of kidney were observed in the experimental group and the wild-type group were more serious than those in the C3a receptor knockout group and the C5a receptor knockout group, but there was no obvious abnormality in the renal histopathology in the negative control group. The relative expression of MCP-1 mRNA in renal tissue of experimental group was higher than that of negative control group, while that of wild-type group was higher than that of C5a receptor knockout group and C3a receptor knockout group. In addition, the relative mRNA expression of IL-6MCP-1 mRNA in C5a receptor knockout group was higher than that in C3a receptor knockout group. Conclusion the loss of C _ 3a and C _ 5a receptors can attenuate the renal injury of IgA nephropathy, and the deletion of C _ 3a receptor is more significant.
【作者单位】： 河南省高等学校临床医学重点学校开放实验室;郑州大学肾脏病研究所;郑州大学医药科学研究院;郑州大学第一附属医院肾内科;
【基金】：国家自然科学基金资助项目(No.81170668)
【分类号】：R692.31

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