前列腺素E1治疗急性肾小管损伤大鼠的实验研究

发布时间：2018-06-26 08:01

本文选题：前列腺素E + 庆大霉素　；参考：《广西医科大学学报》2014年02期

【摘要】：目的:探讨前列腺素E1(PGE1)对急性肾小管损伤大鼠的疗效。方法:将30只Wistar大鼠随机分为3组:实验组、对照组和空白组,每组10只大鼠。实验组、对照组大鼠通过腹腔注射庆大霉素建立急性肾小管损伤模型后,每天给予PGE1或相同体积生理盐水腹腔注射,共14d。空白组大鼠每天给予PGE1相同体积的生理盐水腹腔注射。所有大鼠分别于建模后的1,4,7,14d记录体重、24h尿量,测定血肌酐(SCr)、尿N-乙酰-β-D-氨基葡萄糖甘酶(NAG酶),14d时处死大鼠,取肾组织标本行HE染色观察肾组织形态学改变。结果:建模后1d,与空白组比较,实验组、对照组大鼠24h尿量、SCr、尿NAG酶明显增高,差异有统计学意义(P0.05),但两组间无差异(P0.05);实验组、对照组大鼠体重、24h尿量在建模后相同时间点比较无明显差异(P0.05);实验组、对照组大鼠SCr、尿NAG酶建模后7d达高峰后逐渐下降,与对照组相同时间点比较,实验组大鼠建模后7,14dSCr、尿NAG酶均明显降低(P0.05);建模后14d肾脏病理评级实验组大鼠肾小管损伤较对照组明显减轻(P0.05),小管上皮细胞再生明显。结论:PGE1可降低庆大霉素所致急性肾小管损伤大鼠SCr、尿NAG酶水平,减轻肾组织病理损伤,促进肾小管上皮细胞再生恢复,具有良好的肾脏保护作用。
[Abstract]:Objective: to investigate the effect of prostaglandin E 1 (PGE 1) on acute tubular injury in rats. Methods: thirty Wistar rats were randomly divided into three groups: experimental group, control group and blank group. In the experimental group, the control group rats were treated with PGE1 or the same volume of saline intraperitoneally for 14 days after the acute renal tubular injury model was established by intraperitoneal injection of gentamicin. Rats in the blank group were given intraperitoneal injection of PGE1 of the same volume every day. All the rats were killed on the 14th day after modeling by measuring serum creatinine (SCR) and urine N-acetyl- 尾 -Dglucosaminase (nag) for 14 days. The renal tissue samples were taken to observe the morphological changes of renal tissue by HE staining. Results: 1 day after modeling, compared with the blank group, the 24h urine volume of SCrand nag enzyme in the experimental group and the control group were significantly increased (P0.05), but there was no difference between the two groups (P0.05), while in the experimental group, there was no significant difference between the two groups (P0.05). There was no significant difference in 24h urine volume between the control group and control group at the same time after modeling (P0.05), while in the control group, SCrand urinary nag enzyme reached the peak 7 days after modeling, compared with the control group at the same time point. Compared with the control group, the renal tubular injury in the experimental group was significantly alleviated on the 14th day after modeling (P0.05), and the regeneration of tubular epithelial cells was obvious in the experimental group (P0.05). Conclusion: PGE1 can reduce the level of SCrand nag in urine of rats with gentamicin induced acute renal tubular injury, alleviate the pathological injury of renal tissue, promote the regeneration of renal tubular epithelial cells, and have a good renal protection effect.
【作者单位】：广西医科大学第一附属医院肾内科;广西医科大学第一附属医院儿科;
【基金】：广西壮族自治区卫生厅计划课题资助项目(No.Z2011323)
【分类号】：R692.5

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