糖尿病肾病MKR小鼠蛋白尿与尿中nephrin、PCX含量相关性分析

发布时间：2018-06-28 10:23

本文选题：尿微量白蛋白 + MKR小鼠　；参考：《四川大学学报(医学版)》2015年05期

【摘要】：目的通过检测糖尿病肾病(DN)MKR小鼠足细胞结构蛋白nephrin与足细胞标记蛋白(PCX)在肾组织中的表达量及尿中的含量,分析尿微量白蛋白(UmAlb)与尿中nephrin、PCX蛋白含量的相关性。方法取30只8周龄MKR小鼠随机分为2组(空白组和DN组),空白组以普通饲料喂养,将DN组小鼠单肾切除并加以高脂喂养2个月复制DN模型;另取15只同龄C57野生鼠为正常对照组,以普通饲料喂养。造模前1d、喂养4周及8周后,采用电化学法检测各组小鼠空腹血糖(FBG);喂养8周后,取各组小鼠肾脏组织,用电镜观察肾组织足细胞形态结构;Western blot检测肾组织中nephrin、PCX蛋白表达含量;ELISA法测定尿中UmAlb含量及nephrin、PCX蛋白表达含量。结果电镜结果显示:喂养8周后,与C57组比较,DN组小鼠肾小球足细胞结构损伤明显,DN组FBG升高(P0.01),且肾组织中nephrin、PCX蛋白表达下调,而尿中nephrin、PCX蛋白含量及UmAlb含量均升高(P0.01)。相关分析显示,尿UmAlb与尿中nephrin和PCX蛋白含量呈正相关(相关系数r=0.920,决定系数R2=0.829,P0.05)。结论尿中nephrin、PCX蛋白含量与UmAlb含量呈正相关,足细胞结构蛋白丢失可能是导致糖尿病肾病蛋白尿的机制之一。
[Abstract]:Objective to detect the expression of podocyte structural protein (nephrin) and podocyte marker protein (PCX) in renal tissue and urine in diabetic nephropathy (DN) MKR mice, and to analyze the correlation between urinary microalbumin (UmAlb) and nephrinPCX protein in urine. Methods Thirty 8-week-old MKR mice were randomly divided into two groups (blank group and DN group). Another 15 wild C57 mice of the same age were taken as normal control group and fed with common diet. The fasting blood glucose (FBG) was measured by electrochemical method after feeding for 4 weeks and 8 weeks before the model was made, and the kidney tissue of each group was taken after feeding for 8 weeks. The expression of nephrinnin PCX protein in renal tissue was detected by blot. UmAlb content in urine and nephrinnin PCX protein expression in urine were determined by Elisa. Results the results of electron microscope showed that compared with C57 group, the FBG of glomerular podocytes in DN group was significantly higher than that of C57 group (P0.01), and the expression of nephrinPCX protein was down-regulated in renal tissue, while the contents of nephrininPCX protein and UmAlb in urine were increased (P0.01). The correlation analysis showed that urinary UmAlb was positively correlated with the content of nephrin and PCX protein in urine (r = 0.920, P < 0.05). Conclusion there is a positive correlation between the content of nephrinnin-PCX protein and the content of UmAlb in urine. The loss of podocyte structural protein may be one of the mechanisms leading to proteinuria in diabetic nephropathy.
【作者单位】：湖南中医药大学研究生院;湖南中医药大学;
【基金】：国家自然科学基金(No.81273753) 湖南省自然科学基金(No.12JJ9031) 湖南省高校创新平台开放基金(No.14K069)资助
【分类号】：R587.2;R692.9

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