白藜芦醇后处理对大鼠肾脏缺血再灌注损伤的影响及机制

发布时间：2018-07-13 14:14

【摘要】：目的探讨白藜芦醇后处理对肾脏缺血再灌注(I/R)损伤的影响及其机制。方法将60只大鼠随机分为假手术组、模型对照组、白藜芦醇低剂量组及白藜芦醇高剂量组,每组各15只。模型对照组、白藜芦醇低剂量组及白藜芦醇高剂量组制备急性肾脏I/R模型,假手术组仅行假手术,对双侧肾脏不进行处理。白藜芦醇低剂量组及白藜芦醇高剂量组造模成功后,待肾脏血流恢复后用16号灌胃针进行灌胃给药。假手术组及模型对照组术后立即灌胃0.9%Na Cl溶液2 m L。给药处理24 h后,经腹主动脉取血,检测血清尿素氮(BUN)、肌酐(Cr)水平。取左侧肾脏,配成肾组织匀浆,检测丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。采用HE染色法观察病理学变化,Paller法进行肾小管坏死程度评分。结果白藜芦醇低、高剂量组血清BUN、Cr水平均低于模型对照组(P均0.05),白藜芦醇高剂量组血清BUN、Cr水平均低于白藜芦醇低剂量组(P均0.05)。白藜芦醇低、高剂量组肾组织MDA含量低于模型对照组,SOD活性高于模型对照组(P均0.05);白藜芦醇高剂量组肾组织MDA含量低于白藜芦醇低剂量组,SOD活性高于白藜芦醇低剂量组(P均0.05)。白藜芦醇低、高剂量组肾脏组织病理学改变较模型对照组明显缓解,白藜芦醇高剂量组缓解更明显。白藜芦醇低、高剂量组肾小管坏死程度评分低于模型对照组,白藜芦醇高剂量组低于白藜芦醇低剂量组(P均0.05)。结论白藜芦醇后处理可明显改善肾脏I/R损伤大鼠的肾功能指标、减轻肾脏组织损伤,其机制可能与抑制肾脏组织氧化应激反应有关。
[Abstract]:Objective to investigate the effects of resveratrol postconditioning on renal ischemia reperfusion (I / R) injury and its mechanism. Methods Sixty rats were randomly divided into sham operation group, model control group, resveratrol low dose group and resveratrol high dose group with 15 rats in each group. Model control group, resveratrol low-dose group and resveratrol high-dose group were used to prepare acute renal I / R model. Resveratrol low dose group and resveratrol high dose group were successfully established. In sham operation group and model control group, 0.9% NaCl solution was given immediately after operation. After 24 hours of administration, blood was taken from abdominal aorta and serum urea nitrogen (bun) and creatinine (Cr) were measured. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were measured. The pathological changes were observed by HE staining and the grade of renal tubular necrosis was evaluated by Paller's method. Results the level of bun Cr in serum of high dose group was lower than that of model control group (P 0.05), and that of high dose group of resveratrol group was lower than that of low dose group of resveratrol (P 0.05). The content of MDA in renal tissue of high dose group was lower than that of model control group (P 0.05), and the content of MDA in kidney tissue of high dose group was lower than that of low dose group of resveratrol group (P 0.05), and that of high dose group was higher than that of low dose group (P 0.05). The renal histopathological changes in the high dose group were significantly improved than those in the model control group, and the remission was more obvious in the high dose group than in the model control group. The scores of renal tubular necrosis in high dose group and high dose group were lower than those in model control group (P 0.05), and that in resveratrol high dose group were lower than that in resveratrol low dose group (P 0.05). Conclusion resveratrol post-treatment can significantly improve the renal function index and alleviate the renal tissue damage in rats with I / R injury. The mechanism may be related to the inhibition of oxidative stress reaction in renal tissue.
【作者单位】：泰山医学院;
【基金】：国家级大学生创新创业计划项目(201610439014)
【分类号】：R692

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