hAMSCs移植对鱼胆中毒大鼠肾组织中CD80分子表达的影响

发布时间：2018-07-18 21:32

【摘要】：目的:研究人羊膜间充质干细胞(human amniotic mesenchymal stem cells,hAMSCs)移植治疗鱼胆中毒大鼠急性肾小管坏死(acute tubular necrosis,ATN)肾组织中CD80表达变化,探讨hAMSCs移植治疗鱼胆中毒大鼠ATN的作用机制。方法:采用课题组前期制备的鱼胆灌胃大鼠ATN中毒模型,留取尾静脉注射移植hAMSCs治疗大鼠第3天、7天、14天肾组织石蜡标本,分为对照组(正常大鼠)、模型组(鱼胆中毒大鼠)、移植组(hAMSCs移植治疗大鼠),切片后进行免疫组化染色,光学显微镜下观察CD80表达变化,采集图片测量积分光密度值(IOD),进行半定量分析。结果:(1)hAMSCs移植治疗第3天、7天、14天,对照组和移植组皮质区肾小球CD80表达无明显差异(P0.05),模型组第3天CD80表达量高于第7天、14天(P0.05),第7天、14天与对照组和移植组无明显差异(P0.05)。(2)hAMSCs移植治疗第3、7天,模型组皮质区肾小管上皮细胞CD80表达量最低(P0.05),第14天与对照组无明显差异(P0.05),移植组第3天表达量最高(P0.05),第7天、14天与对照组无明显差异(P0.05)。(3)hAMSCs移植治疗第3天、7天、14天,对照组髓质区肾小管上皮细胞未见CD80表达(P0.05),模型组第3天髓质区CD80表达量高于第7天、14天(P0.05),第7天表达量明显减少,但高于第14天(P0.05),hAMSCs移植治疗第3天,模型组高于移植组(P0.05),移植组可见少量表达,但高于对照组(P0.05);结论:hAMSCs移植治疗ATN大鼠可能通过抑制肾小球及肾小管上皮细胞CD80的表达,进而减少T细胞的激活,减轻炎症反应。
[Abstract]:Aim: to study the changes of CD80 expression in renal tissue of rats with acute tubular necrosis (acute tubular necrosis) treated with human amniotic mesenchymal stem cells (human amniotic mesenchymal stem cells) and to explore the mechanism of transplantation of human amniotic mesenchymal stem cells (hAMSCs) in the treatment of fish bile duct poisoning rats. Methods: the rat model of ATN poisoning was prepared by fish gall instillation in the early stage of the study group. The rats were treated by tail vein injection of hAMSCs on day 3 and day 7, and the paraffin tissues of renal tissue on day 14 were collected. The rats were divided into control group (normal rats), model group (fish bile poisoning rats) and transplantation group (hAMSCs transplantation treated rats). The integrated optical density (IOD) was measured by collecting pictures, and semi-quantitative analysis was carried out. Results: (1) hAMSCs were transplanted on day 3 and day 7 and day 14, respectively. There was no significant difference in the expression of CD80 between the control group and the transplantation group (P0.05). The expression of CD80 in the model group was higher on the 3rd day than on the 7th day (P0.05), and on the 7th day, there was no significant difference between the control group and the transplantation group (P0.05) on the 3rd day after transplantation (P0.05), and on the 7th day after transplantation, there was no significant difference in the expression of CD80 between the control group and the transplantation group (P0.05). The expression of CD80 in renal tubular epithelial cells in the model group was the lowest (P0.05), but there was no significant difference between the model group and the control group on the 14th day (P0.05). The expression of CD80 on the 3rd day (P0.05) in the transplantation group was the highest (P0.05), and there was no significant difference (P0.05) on the 14th day (P0.05) in the 3rd day and the 14th day in the transplantation group (P0.05). CD80 expression was not found in renal tubular epithelial cells in the medullary region of the control group (P0.05). The expression of CD80 in the medullary area of the model group was higher on the 3rd day than on the 7th day (P0.05), and decreased significantly on the 7th day, but higher than that on the 14th day (P0.05) on the 3rd day after transplantation of hAMSCs. The expression of CD80 in the model group was higher than that in the transplantation group (P0.05), but it was higher in the transplantation group than that in the control group (P0.05). Conclusion the expression of CD80 in glomeruli and renal tubular epithelial cells may be inhibited by the transplantation of 10% hAMSCs in ATN rats, and then the activation of T cells and inflammation can be alleviated.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R595.7;R692.5

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