脂联素在前列腺癌中作用的初步研究

发布时间：2018-07-21 14:56

【摘要】：目的:研究血清脂联素水平与前列腺癌发生的关系以及脂联素对前列腺癌细胞生物学特性的影响。方法:采用病例对照试验回顾性分析45例前列腺癌患者(病例组)和50例非前列腺癌患者(对照组)血清脂联素水平和前列腺癌发生之间的关系。利用慢病毒载体构建脂联素受体缺陷的稳转细胞株,用脂联素干预前列腺癌细胞系并通过CCK-8实验、流式细胞实验、成管实验和划痕实验分析脂联素作用升高和降低对PC3细胞生物学行为的影响。结果:与对照组患者[(6.35±4.43)μg/ml]比较,病例组血清脂联素水平[(3.49±2.70)μg/ml]降低,并且脂联素水平与Gleason评分高低呈负相关(r=-0.504,P0.01)。脂联素抑制PC3细胞的增殖,迁移,抑制细胞进入S期,并抑制血管形成,而在脂联素受体缺陷的情况下,PC3细胞反而具有更强的增殖、迁移和促血管形成能力。结论:血清脂联素下降或脂联素受体缺陷可能是促进前列腺癌发生的因素。
[Abstract]:Aim: to study the relationship between serum adiponectin level and prostate cancer and the effect of adiponectin on the biological characteristics of prostate cancer cells. Methods: serum adiponectin levels in 45 patients with prostate cancer (case group) and 50 patients without prostate cancer (control group) were analyzed retrospectively by case-control trial. A stable cell line with adiponectin receptor deficiency was constructed by using lentivirus vector. Adiponectin was used to interfere with prostate cancer cell line. The effects of adiponectin on the biological behavior of PC3 cells were analyzed by tube forming test and scratch test. Results: compared with the control group [(6.35 卤4.43) 渭 g/ml], the serum adiponectin level [(3.49 卤2.70) 渭 g/ml] was decreased, and there was a negative correlation between the adiponectin level and the Gleason score (r = -0.504, P0.01). Adiponectin inhibited the proliferation and migration of PC3 cells, inhibited cell entry into S phase and inhibited angiogenesis. However, when adiponectin receptor was deficient, PC3 cells had stronger ability of proliferation, migration and angiogenesis. Conclusion: the decrease of serum adiponectin or the deficiency of adiponectin receptor may be the factors that promote the development of prostate cancer.
【作者单位】：上海交通大学医学院附属第九人民医院泌尿外科;中山大学孙逸仙纪念医院泌尿外科;
【基金】：国家自然科学基金(81172450,81402089) 上海浦东新区卫计委资助项目(PWZxq2014-11)~~
【分类号】：R737.25

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