西罗莫司和环孢素对常染色体隐性遗传性多囊肾病大鼠疾病进展过程的影响

发布时间：2018-07-24 08:17

【摘要】：目的观察西罗莫司和环孢素(cyclosporin,Cs A)对常染色体隐性遗传性多囊肾病(autosomal recessive polycystic kidney disease,ARPKD)大鼠疾病进程的影响。方法 20只4周龄ARPKD模型PCK大鼠,雌雄各半,采用成组设计随机分为西罗莫司组(n=6)、环孢素组(n=8)和对照组(n=6),分别每天以西罗莫司稀释液、环孢素稀释液和无药物的饮用水灌胃,每周测体重,分别于4、6、8、10周龄内眦静脉取血监测肾功能,分别在4、8、12周龄行双肾超声观察囊肿数量及大小,12周龄时全部处死,取双肾称重,并行切片HE染色观察囊肿体积密度(cyst volume density,CVD)。结果 3组大鼠实验过程中均无死亡,体重均增加,但3组间无显著差异(P0.05)。12周龄时西罗莫司组超声下肾脏体积、肾脏内囊肿数和最大囊肿最大截面面积均明显低于对照组[(1.65±0.25)cm~3vs.(2.00±0.36)m~3,q=3.426,P0.05;(31.75±6.57)个vs.(43.08±10.28)个,q=3.638,P0.05;(0.0828±0.0420)cm~2vs.(0.1373±0.0536)cm~2,q=3.966,P0.05];环孢素组超声下肾脏体积、肾脏内囊肿数和最大囊肿最大截面面积与对照组无显著差异(P0.05)。西罗莫司组、环孢素组和对照组在双肾质量、双肾质量/体重比(2-kidney/total body weight ratio,2K/TBW)、10周龄时血清BUN和Cr以及CVD均无显著差异(P0.05)。结论西罗莫司和Cs A对PKD大鼠疾病的进程未表现出明显的影响。
[Abstract]:Objective to investigate the effects of sirolimus and cyclosporin A on the progression of autosomal recessive polycystic kidney disease (autosomal recessive polycystic kidney disease) in rats. Methods Twenty 4-week-old ARPKD model PCK rats were randomly divided into three groups: sirolimus group (n = 6), cyclosporine group (n = 8) and control group (n = 6). The body weight was measured weekly, and the renal function was monitored by blood collection from the medial canthus vein at the age of 8 weeks and 10 weeks. The number and size of the cysts were observed by ultrasonography at the age of 48 ~ 12 weeks and all the cysts were killed at the age of 12 weeks. The two kidneys were taken to weigh the cysts, and the volume density of the cysts was observed by HE staining. Results there was no death and weight gain in all the three groups, but there was no significant difference among the three groups (P0.05). The number of cysts in the kidney and the maximum area of the maximum cysts in the kidney were significantly lower than those in the control group [(1.65 卤0.25) cm ~ (3) ~ (3) vs. (2.00 卤0.36) mg ~ (3) Q ~ (3) ~ (3) ~ (26) (P ~ (0.05); (31.75 卤6.57) Vs. (43.08 卤10.28) ~ (3.638) P _ (0.05); (0.0828 卤0.0420) cm ~ (2) C _ (2) vs (0.1373 卤0.0536) cm ~ (2) Q _ (3.966) P _ (0.05); there was no significant difference between cyclosporin group and the control group in the number of renal cysts and the maximum cysts area (P < 0.05). There was no significant difference in serum BUN, Cr and CVD between sirolimus group, cyclosporine group and control group at 10 weeks of age (P0.05). Conclusion sirolimus and CSA have no significant effect on the progression of PKD rats.
【作者单位】：北京大学第三医院泌尿外科;北京大学第三医院病理科;北京大学第三医院超声诊断科;北京大学第三医院普通外科;
【基金】：卫生部国际交流与合作中心项目(项目编号:IHECC08-201205)
【分类号】：R692

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