阿托伐他汀对急性肾损伤大鼠的肾保护作用及炎症因子表达的影响

发布时间：2018-07-26 20:32

【摘要】：目的探讨阿托伐他汀(Ato)对急性肾损伤(AKI)大鼠的肾保护作用及炎症因子表达的影响。方法选取SD大鼠,采用皮下连续3 d注射庆大霉素制备中毒型AKI大鼠模型,20只AKI大鼠于第4天接受10 mg/kg Ato灌胃处理,1次/d,连续7 d(Ato组);20只AKI大鼠(AKI组)及正常SD大鼠(对照组)仅接受同等量蒸馏水灌胃1 w。实验结束后收集各组大鼠的血液、尿液用于检测肾功能[血清肌酐(Scr)、尿素氮(BUN)和胱抑素C(Cys C)]、尿中性粒细胞明胶酶相关脂质运载蛋白(NGAL)及血清炎症因子[白介素(IL)-6、肿瘤坏死因子(TNF)-α和C-反应蛋白(CRP)]水平,同时取肾组织用于检测其血管活性物质[一氧化氮(NO)和内皮素(ET)-1]及氧化应激[超氧化物歧化酶(SOD)和丙二醛(MDA)]水平。结果与对照组大鼠比较,AKI组的Scr、Cys C、BUN、尿NGAL及肾组织NO、ET-1及MDA水平均升高,肾组织SOD活性降低,以上提示AKI造模成功,AKI大鼠肾损伤严重。Ato组的肾功能指标、尿NGAL及肾组织NO、ET-1和MDA水平均低于AKI组(P0.05),肾组织SOD活性高于AKI组(P0.05);但与正常组的差异仍有统计学意义(P0.05)。结论 Ato能改善庆大霉素制备中毒型AKI大鼠模型的肾功能,可能与减轻炎症反应及氧化应激有关,且Ato在改善AKI症状上有一定的应用前景。
[Abstract]:Objective to investigate the effects of Atto vastatin (Ato) on renal protection and inflammatory factor expression in (AKI) rats with acute renal injury. Methods SD rats were selected, Twenty AKI rats were treated with 10 mg/kg Ato intragastric administration of 10 mg/kg Ato for 1 / d on the 4th day. 20 AKI rats (AKI group) and 20 normal SD rats (control group) were given subcutaneous injection of gentamicin for 3 days. The rats were treated with the same amount of distilled water for 1 week. At the end of the experiment, the blood of the rats in each group was collected, Urine was used to detect the levels of renal function [serum creatinine (Scr), urea nitrogen (BUN) and cystatin C (Cys C)], urinary neutrophil gelatinase-associated lipid carrier protein (NGAL) and serum inflammatory factor [interleukin (IL)-6, tumor necrosis factor (TNF)-伪 and C-reactive protein (CRP)]. Renal tissue was also taken to detect the levels of vasoactive substances [nitric oxide (NO) and endothelin (ET) -1] and oxidative stress (superoxide dismutase (SOD) and malondialdehyde (MDA). Results compared with the control group, the levels of NGAL in urine, NON-ET-1 and MDA in renal tissue were increased, and the activity of SOD in renal tissue was decreased in AKI group, which indicated that the renal function index of renal injury was serious in AKI group. The levels of urinary NGAL and NON-ET-1 and MDA in renal tissue were lower than those in AKI group (P0.05), and the activity of SOD in renal tissue was higher than that in AKI group (P0.05), but there was still significant difference compared with normal group (P0.05). Conclusion Ato can improve renal function of AKI rats induced by gentamicin, which may be related to reducing inflammatory reaction and oxidative stress, and Ato has a certain application prospect in improving the symptoms of AKI.
【作者单位】：华中科技大学同济医学院附属普爱医院肾病内科;
【基金】：湖北省自然科学基金面上项目(2014CFC1047)
【分类号】：R692.5

【相似文献】