Boule基因在小鼠精子发生过程中的动态表达

发布时间：2018-07-27 11:02

【摘要】：目的:研究雄性不育相关基因Boule在小鼠出生后首个精子发生波中的动态表达,探讨Boule在精子发生过程中的调控作用。方法:运用免疫印迹法分析BOULE蛋白在多组织中的表达情况。运用实时荧光定量、免疫组织化学和免疫荧光技术检测Boule m RNA和BOULE蛋白在雄性小鼠出生后首个精子发生波各个时间点中的表达。结果:1免疫印迹法显示BOULE蛋白在睾丸中特异性高表达,在出生后16 d睾丸可被检测到,并在随后的发育中睾丸和成年睾丸持续表达;2实时荧光定量方法证实Boule m RNA在雄鼠出生后14 d睾丸中可被检测到,20 d呈现表达高峰;3免疫组织化学显示在精母细胞及圆形精子细胞胞质中存在BOULE蛋白阳性信号。结论:Boule在精子发生过程中的表达是在发育水平精密调控的,在粗线期精母细胞和圆形精子细胞阶段的特异表达提示Boule可能在精母细胞进程和圆形精子细胞中发挥作用。
[Abstract]:Aim: to investigate the dynamic expression of male sterility related gene (Boule) in the first spermatogenesis wave in mice and to explore the regulatory role of Boule in spermatogenesis. Methods: the expression of BOULE protein in multiple tissues was analyzed by Western blot. The expression of Boule m RNA and BOULE proteins in the first spermatogenesis wave of male mice was detected by real-time fluorescence quantitative, immunohistochemical and immunofluorescence techniques. Results the specific expression of BOULE protein was high in testis by Western blotting, and could be detected in testis 16 days after birth. The results of real-time fluorescence quantitative analysis of the continuous expression of Ts2 in the developing testis and adult testis confirmed that Boule m RNA could be detected in the testis of male mice at 14 days postnatal, and the expression peak was detected in the testis of male mice at day 20. Immunohistochemical staining showed that the expression peak was observed in the fine spermatozoa of spermatozoa. Positive signals of BOULE protein were found in cytoplasm of spermatozoa and round spermatozoa. Conclusion the specific expression of Boule during spermatogenesis is precisely regulated at the developmental level. The specific expression of Boule in spermatocytes and round spermatozoa suggests that Boule may play a role in spermatocyte progression and round spermatocyte.
【作者单位】：南京医科大学生殖医学国家重点实验室;
【基金】：国家自然科学基金资助(81270737) 江苏省自然科学基金面上项目(BK20122838)
【分类号】：R698.2;Q78

【相似文献】