G蛋白信号转导调节因子4在肾癌组织中的表达及意义

发布时间：2018-08-02 08:38

【摘要】：目的探讨肾细胞癌(RCC)组织中G蛋白信号转导调节因子4(RGS4)的表达变化及其与RCC患者临床病理特征的关系。方法收集120例RCC患者手术切除的癌组织及癌旁组织,采用免疫组化染色及Western blot法检测RGS4蛋白表达,RT-PCR法检测RGS4 mRNA表达,分析不同临床病理特征的RCC患者癌组织RGS4蛋白表达变化。结果免疫组化染色示,RGS4特异性表达于肾细胞的细胞质中,在癌旁组织中为浅黄色,在癌组织中为深黄色。癌旁组织、RCC癌组织中RGS4蛋白表达阳性率分别为24.2%、37.5%,RCC癌组织中RGS4蛋白表达阳性率高于癌旁组织(P0.05)。癌旁组织、RCC癌组织RGS4蛋白表达量分别为0.66±0.03、1.02±0.03,RCC癌组织RGS4蛋白表达量高于癌旁组织(P0.05)。癌旁组织、RCC癌组织RGS4 mRNA表达量分别为0.93±0.04、1.67±0.04,RCC癌组织RGS4 mRNA表达量高于癌旁组织(P0.05)。中度及重度浸润的RCC患者RGS4表达率显著高于轻度浸润的患者(P0.05)。结论 RCC癌组织中RGS4表达增高,并随浸润程度增加表达率显著升高,参与RCC的发生发展。
[Abstract]:Objective to investigate the expression of G protein signal transduction regulatory factor 4 (RGS4) in renal cell carcinoma (RCC) and its relationship with the clinicopathological features of RCC. Methods the expression of RGS4 mRNA was detected by immunohistochemical staining and Western blot method in 120 patients with RCC. The expression of RGS4 protein was detected by RT-PCR in RCC patients with different clinicopathological features. The expression of RGS4 protein was detected by immunohistochemical staining and RT-PCR. The changes of RGS4 protein expression in the tissues of RCC patients with different clinicopathological features were analyzed. Results Immunohistochemical staining showed that RGS4 was specifically expressed in the cytoplasm of renal cells, which was yellowish in paracancerous tissues and dark yellow in cancerous tissues. The positive rate of RGS4 protein expression in paracancerous tissues was higher than that in paracancerous tissues (P 0.05). The expression of RGS4 protein in paracancerous tissues was higher than that in paracancerous tissues (P 0.05). The expression of RGS4 mRNA in paracancerous tissues was higher than that in paracancerous tissues (0. 93 卤0. 04) and 1. 67 卤0. 04 respectively (P0.05). The expression rate of RGS4 in RCC patients with moderate and severe infiltration was significantly higher than that with mild invasion (P0.05). Conclusion the expression of RGS4 in RCC carcinoma is increased and the expression rate is increased with the degree of invasion, which is involved in the occurrence and development of RCC.
【作者单位】： 河北大学附属医院;华北石油总医院;
【分类号】：R737.11

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