HOXA13对肾小管上皮细胞间充质转化和BMP-7表达的影响

发布时间：2018-08-02 12:08

【摘要】：目的探讨HOXA13对人血清白蛋白(HSA)诱导的人肾小管上皮细胞(HKC)间充质转分化(EMT)和骨形成蛋白7(BMP-7)表达的影响。方法体外培养人肾小管上皮细胞(HKC)经20 mg/ml人血清白蛋白作用48 h后,采用Real-Time PCR和免疫印迹法分别检测人肾小管上皮细胞角蛋白(CK)、波形蛋白(Vimentin)和HOXA13 mRNA和蛋白表达。应用脂质体转染技术上调人肾小管上皮细胞HOXA13表达,观察HOXA13不同表达水平对人血清白蛋白超载诱导CK、Vimentin、BMP-7表达改变的影响。结果 20 mg/ml HSA刺激HKC细胞48 h后,HKC出现EMT样改变:CK表达下降(P0.01)、Vimentin表达增高(P0.01);HSA超载同时使HKC细胞内HOXA13、BMP-7表达下降。基因转染上调HKC细胞内HOXA13表达后,能够显著抑制HSA超载诱导的EMT发生(P0.05)和BMP-7表达下降(P0.05)。结论 HOXA13拮抗白蛋白超载诱导肾小管上皮细胞发生EMT的作用可能与HOXA13上调BMP-7表达有关。
[Abstract]:Objective to investigate the effects of HOXA13 on the expression of (EMT) and bone morphogenetic protein 7 (BMP-7) in human renal tubular epithelial cells induced by human serum albumin (HSA) (HSA). Methods cultured human renal tubular epithelial cells (HKC) were incubated with 20 mg/ml human serum albumin for 48 h. The expression of (CK), vimentin (Vimentin), HOXA13 mRNA and protein in human renal tubular epithelial cells was detected by Real-Time PCR and Western blot, respectively. Liposome transfection technique was used to up-regulate the expression of HOXA13 in human renal tubular epithelial cells and to observe the effect of different levels of HOXA13 expression on the expression of Vimentin BMP-7 induced by human serum albumin overload. Results after stimulation of HKC cells for 20 mg/ml HSA for 48 h, EMT like changes were observed in HKC cells. The expression of Vimentin was increased (P0.01) and the expression of HOXA13 BMP-7 in HKC cells was decreased at the same time when the expression of Vimentin was increased (P0.01). After up-regulation of HOXA13 expression in HKC cells, gene transfection significantly inhibited EMT induced by HSA overload (P0.05) and decreased BMP-7 expression (P0.05). Conclusion the antagonistic effect of HOXA13 on the production of EMT in renal tubular epithelial cells induced by albumin overload may be related to the up-regulation of BMP-7 expression by HOXA13.
【作者单位】：中南大学湘雅二医院小儿肾脏病研究室;
【分类号】：R692

【相似文献】