CircCD44-360与circCD44-594在膀胱癌中的表达及临床意义

发布时间：2018-08-09 09:05

【摘要】：目的:本研究就circCD44-360(hsa_circ_0021727)与circCD44-594(hsa_circ_0021728)在膀胱癌及癌旁组织中的表达情况,及它们对CD44基因表达的影响和相关性分析进行研究,旨在为膀胱癌的诊断和靶向治疗提供新的方向。方法:收集膀胱癌及癌旁组织的临床标本,处理标本后提取膀胱癌及癌旁组织的总RNA,反转录获取目的RNA,进行实时定量PCR,检测circCD44-360与circCD44-594的表达差异,并分析其分别同亲本基因CD44的信使RNA(messenger RNA,mRNA)的关系进而找出其调节机制。结果:1 CircCD44-360与circCD44-594在膀胱癌组织的表达均上调。PCR结果显示,与癌旁组织相比,circCD44-360与circCD44-594在膀胱癌组织中的表达水平显著升高。说明circCD44-360与circCD44-594在膀胱癌组织中的表达水平均显著上调。2 CD44mRNA在膀胱癌组织的表达上调。PCR结果显示,与癌旁组织相比,CD44mRNA在膀胱癌组织中的表达水平显著升高。说明CD44mRNA在膀胱癌组织中的表达水平均显著上调。3 CircCD44-360与circCD44-594均同其亲本基因CD44的表达呈线性正相关关系。利用PCR结果进行统计学分析,circCD44-360与其亲本基因CD44的Pearson's correlation线性相关关系,R=0.5702,P=0.0087,呈正相关。另一个环状circCD44-594与其亲本基因CD44的Pearson's correlation线性相关关系,R=0.4474,P=0.0420,呈正相关。结论:1 CircCD44-360在膀胱癌组织中表达上调。2 CircCD44-594在膀胱癌组织中表达上调。3在膀胱癌中,circCD44-360、circCD44-594和CD44的表达呈正相关关系,它们可能通过调节CD44基因的表达促进膀胱癌的发生和发展。
[Abstract]:Objective: To study the expression of circCD44-360 (hsa_circ_0021727) and circCD44-594 (hsa_circ_0021728) in bladder cancer and para cancer tissues, and their influence on the expression of CD44 gene and the correlation analysis. The purpose of this study is to provide new directions for the diagnosis and target therapy of bladder cancer. Methods: to collect bladder cancer and para cancer tissue. After treating specimens, the total RNA of bladder cancer and para cancer tissue was extracted, RNA was obtained by reverse transcription, and the real-time quantitative PCR was used to detect the difference between circCD44-360 and circCD44-594, and the regulation mechanism of the RNA (messenger RNA, mRNA) with the parent gene CD44, respectively, was analyzed. The results were as follows: 1 CircCD44-360 and circC. The expression of D44-594 in bladder cancer tissues was up regulated by.PCR, and the expression level of circCD44-360 and circCD44-594 in bladder cancer tissue was significantly higher than that of para cancerous tissue. The expression level of circCD44-360 and circCD44-594 in bladder cancer tissues was significantly up-regulated and the expression of.2 CD44mRNA in bladder cancer tissue was up to.PCR results. The expression level of CD44mRNA in bladder cancer tissue was significantly higher than that of para cancerous tissue. It indicated that the expression level of CD44mRNA in bladder cancer tissues was significantly up-regulated and that the expression of.3 CircCD44-360 and circCD44-594 were linearly correlated with the expression of their parent gene CD44. Statistical analysis of PCR knot fruit, circCD44-360 and its parents The Pearson's correlation linear correlation of gene CD44, R=0.5702, P=0.0087, is positively correlated. The other circular circCD44-594 is correlated with the Pearson's correlation linear correlation of the parent gene CD44, R=0.4474, P=0.0420. Conclusion: 1 CircCD44-360 in bladder cancer tissues is up regulated in bladder cancer tissue. The expression of.3 in bladder cancer is positively correlated with the expression of circCD44-360, circCD44-594 and CD44. They may promote the development and development of bladder cancer by regulating the expression of CD44 gene.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.14

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