百里醌对肾缺血再灌注损伤大鼠的作用及机制

发布时间：2018-08-14 19:24

【摘要】：目的探讨百里醌对肾缺血再灌注损伤大鼠的抗氧化及线粒体保护作用。方法检测百里醌对大鼠血清尿素氮(BUN)和肌酐(Cr)、肾脏病理形态、肾组织匀浆中丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、乳酸(LD)、一氧化氮(NO)的水平以及肾组织线粒体膜电位和线粒体组分中的MDA、GPx、GSH、SOD含量和ATP酶活性变化的影响。结果百里醌可明显减少BUN、Cr、LD、NO、线粒体膜电位、肾组织和线粒体组分中MDA表达以及肾组织病理形态改变,但可明显增加ATP酶活性以及肾组织和线粒体组分中CAT、GPx与SOD的表达。结论百里醌预处理减轻肾缺血再灌注损伤可能与其抗氧化应激和保护线粒体的作用相关。
[Abstract]:Objective to investigate the antioxidant and mitochondrial protective effects of thyriquinone on renal ischemia reperfusion injury in rats. Methods the renal histopathology of serum urea nitrogen (BUN) and creatinine (Cr), (Cr),) in rats were determined by thymidone. The levels of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), lactate (LD), nitric oxide (NO) in renal homogenate and the mitochondrial membrane potential in renal tissue and the content of (CAT), Glutathione glutathione peroxidase (GPx), and ATP in renal tissue homogenate The effect of changes in activity. Results Thyraquinone could significantly decrease the expression of MDA, mitochondrial membrane potential, MDA expression in kidney and mitochondria, and the pathological changes of renal tissue, but significantly increased the activity of ATP and the expression of ATP and SOD in kidney and mitochondria. Conclusion the protective effect of thyriquinone preconditioning on renal ischemia-reperfusion injury may be related to antioxidant stress and mitochondria protection.
【作者单位】：四川省宜宾市第一人民医院肾内科;电子科技大学临床医学系;湖北省武汉市妇女儿童医疗保健中心肾内科;
【基金】：国家自然科学基金资助项目(编号:81500575)
【分类号】：R692

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