MicroRNA-224靶向CNNM1抑制前列腺癌血管生成的实验研究
发布时间：2018-08-19 20:59
【摘要】：目的研究microR NA-224(miR-224)对前列腺癌生化复发及血管生成的影响。方法生物信息学分析及荧光素酶报告实验预测细胞周期调节蛋白1(CNNM1)受miR-224负性调控。Taylor前列腺癌数据库分析、验证CNNM1、miR-224的表达关系及其与前列腺癌生化复发的相关性。前列腺癌PC3细胞体外培养及动物体内成瘤实验研究CNNM1、miR-224表达对前列腺癌微血管生成标志物CD31的影响。结果 CNNM1表达受miR-224靶向调节,前列腺癌组织中CNNM1与miR-224的表达呈负相关(P0.05)。miR-224与前列腺癌的生化复发呈负相关(P0.05),CNNM1与前列腺的生化复发呈正相关(P0.05);在过表达miR-224的PC3细胞株内,CNNM1和CD31的表达量下降;CNNM1过表达能促进CD31的生成。前列腺癌细胞裸鼠体内成瘤组织免疫组织化学法染色提示,miR-224过表达能抑制前列腺癌组织内微血管形成。结论 miR-224通过靶向调控CNNM1表达,抑制前列腺癌微血管形成,控制前列腺癌生化复发。
[Abstract]:Objective to study the effect of microR NA-224 (miR-224) on the biochemical recurrence and angiogenesis of prostate cancer. Methods bioinformatics analysis and luciferase report assay were used to predict the relationship between the expression of cell cycle regulator protein 1 (CNNM1) and the biochemical recurrence of prostate cancer. Study on the effect of CNNM1 miR-224 expression on prostate cancer microangiogenesis marker CD31 in vitro and in vivo tumorigenesis of prostate cancer PC3 cells. Results the expression of CNNM1 was regulated by miR-224. There was a negative correlation between the expression of CNNM1 and miR-224 in prostate cancer tissues (P0.05). MiR-224 was negatively correlated with the biochemical recurrence of prostate cancer (P0.05) and CNNM1 was positively correlated with the biochemical recurrence of prostate (P0.05), and the expression of CNNM1 and CD31 decreased in PC3 cell lines that overexpressed miR-224. Danone promotes the formation of CD31. Immunohistochemical staining showed that the overexpression of mmiR-224 could inhibit the formation of microvessel in prostate cancer tissues in nude mice. Conclusion miR-224 can inhibit the microangiogenesis of prostate cancer and control the recurrence of prostate cancer by targeting the expression of CNNM1.
【作者单位】： 中山大学附属中山医院泌尿外科;
【基金】：中国博士后科学基金(No:2016M590842) 广东省医学科研基金(No:A2016052) 广东省中山市科技计划(No:2016B1028)
【分类号】：R737.25
，
本文编号：2192814
[Abstract]:Objective to study the effect of microR NA-224 (miR-224) on the biochemical recurrence and angiogenesis of prostate cancer. Methods bioinformatics analysis and luciferase report assay were used to predict the relationship between the expression of cell cycle regulator protein 1 (CNNM1) and the biochemical recurrence of prostate cancer. Study on the effect of CNNM1 miR-224 expression on prostate cancer microangiogenesis marker CD31 in vitro and in vivo tumorigenesis of prostate cancer PC3 cells. Results the expression of CNNM1 was regulated by miR-224. There was a negative correlation between the expression of CNNM1 and miR-224 in prostate cancer tissues (P0.05). MiR-224 was negatively correlated with the biochemical recurrence of prostate cancer (P0.05) and CNNM1 was positively correlated with the biochemical recurrence of prostate (P0.05), and the expression of CNNM1 and CD31 decreased in PC3 cell lines that overexpressed miR-224. Danone promotes the formation of CD31. Immunohistochemical staining showed that the overexpression of mmiR-224 could inhibit the formation of microvessel in prostate cancer tissues in nude mice. Conclusion miR-224 can inhibit the microangiogenesis of prostate cancer and control the recurrence of prostate cancer by targeting the expression of CNNM1.
【作者单位】： 中山大学附属中山医院泌尿外科;
【基金】：中国博士后科学基金(No:2016M590842) 广东省医学科研基金(No:A2016052) 广东省中山市科技计划(No:2016B1028)
【分类号】：R737.25
，
本文编号：2192814
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