血压的性别差异与小鼠肾脏钠离子通道的关系

发布时间：2018-08-20 17:01

【摘要】：目的研究雌雄小鼠的血压、肾脏尿钠排泄以及相关的肾脏钠离子通道和信号通路,初步探讨血压的性别差异与肾脏钠离子通道的关系。方法选取成年雌雄小鼠,用无创血压仪检测小鼠血压,火焰分光光度计测量小鼠血、尿钠离子浓度,Real-time PCR和Western-Blot检测小鼠肾脏钠氯同向转运体(NCC)、钠钾氯同向转运体(NKCC2)、上皮钠离子通道(ENa C)的表达水平,以及上游的缺乏赖氨酸的丝氨酸苏氨酸蛋白激酶(WNK激酶)的mRNA水平。结果雌鼠血压低于雄鼠,尿钠排泄高于雄鼠,NCC、NKCC2的mRNA和蛋白水平均明显增加,ENa C无明显差异,WNK4显著升高,WNK1无差异。结论雌鼠血压低于雄鼠可能是由雌鼠尿钠排泄增加导致,WNK4-NCC/NKCC2信号通路参与其调控。
[Abstract]:Objective to study blood pressure, urinary sodium excretion, renal sodium channel and signal pathway in male and female mice, and to explore the relationship between sex difference of blood pressure and renal sodium channel. Methods the blood pressure of adult female and male mice was measured by noninvasive blood pressure analyzer, and the blood of mice was measured by flame spectrophotometer. Urinary sodium concentration real-time PCR and Western-Blot were used to detect the expression of (NCC), sodium potassium chloride cotransporter (NKCC2) and epithelial sodium ion channel (ENa C) (ENa C) in kidney of mice. And upstream lysine-deficient serine threonine protein kinase (WNK kinase) mRNA levels. Results the blood pressure of female rats was lower than that of male rats, and urinary sodium excretion was higher than that of male rats. The levels of mRNA and protein of NCC-NK2 in male rats were significantly higher than those in male rats. There was no significant difference in the levels of mRNA and protein between female rats and male rats. WNK4 significantly increased the level of WNK1. Conclusion the blood pressure of female rats is lower than that of male rats, which may be due to the increase of urinary sodium excretion in female rats, resulting in the regulation of WNK4-NCC-NKCC2 signaling pathway.
【作者单位】：温州医科大学检验医学院生命科学学院浙江省遗传学重点实验室;
【基金】：国家自然科学基金(81300709) 浙江省自然科学基金(LY13H070007)
【分类号】：R692;R544.1

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