红细胞生成素抑制活性氧诱导的红细胞衰亡
发布时间：2018-08-22 13:11
【摘要】：目的:观察红细胞生成素(erythropoietin,EPO)对过氧化氢(H_2O_2)刺激后红细胞衰亡(eryptosis)和红细胞中活性氧簇(reactive oxygen species,ROS)生成的影响,并探讨其可能机制。方法:将1%健康人红细胞悬液在以下3组不同的体外培养液中孵育:对照组(C组,培养基为PBS液)、H_2O_2组(H组,培养基为H_2O_2终浓度100μmol/L的PBS液)和EPO组(E组,培养基为H_2O_2终浓度100μmol/L、EPO终浓度2×10~4U/L的PBS液)。分别在孵育24 h和60 h时,留取红细胞以备检测。使用流式细胞术检测红细胞的衰亡率、红细胞内ROS和红细胞内钙离子浓度(_i~([Ca2+])),观察各检测指标的变化并分析其相关性。结果:红细胞衰亡率在C组随孵育时间延长而增加,在相同观察时点,H组较C组明显增加(P0.01),E组较H组明显降低(P0.01)。H组红细胞的ROS生成较C组明显增多,_i~([Ca2+])较C组明显升高(P0.01);E组红细胞的ROS生成较H组明显减少,_i~([Ca2+])较H组明显降低(P0.05或P0.01)。结论:H_2O_2诱导健康红细胞加速衰亡,而EPO可以抑制H_2O_2诱导的红细胞衰亡,其机制可能与抗氧化及_i~([Ca2+])的改变有关。
[Abstract]:Aim: to investigate the effects of erythropoietin (EPO) on the production of (eryptosis) and reactive oxygen species (Ros) in erythrocyte induced by hydrogen peroxide (H_2O_2), and to explore its possible mechanism. Methods: 1% healthy human erythrocyte suspension was incubated in the following three groups in vitro: control group (group C, PBS solution) and group H _ 2O _ 2 (H group, PBS solution with 100 渭 mol/L H_2O_2 final concentration) and EPO group (E group). The medium was PBS solution with final concentration of 100 渭 mol / L H_2O_2 and final concentration of 2 脳 10~4U/L. After incubation for 24 h and 60 h, red blood cells were collected for detection. The decay rate of red blood cells, the concentration of ROS and calcium in red blood cells were detected by flow cytometry. Results: erythrocyte decay rate increased with the increase of incubation time in group C. At the same observation point, the ROS production of erythrocytes in group H was significantly higher than that in group C (P0.01). The ROS production of erythrocytes in group E was significantly lower than that in group H (P0.01). The ROS production of erythrocytes in group H was significantly higher than that in group C (P0.01). The ROS production of erythrocyte in group E was significantly higher than that in group C (P0.01). The ROS production of erythrocyte in group E was significantly lower than that in group H, and that in group H was significantly lower than that in group H. Group A was significantly lower (P0.05 or P0.01). Conclusion\%\
【作者单位】： 山东大学第二医院肾内科;
【基金】：山东大学第二医院种子基金资助项目(No.S2015010001) 山东省自然科学基金资助项目(No.ZR2014HM045)
【分类号】：R692.5
本文编号：2197193
[Abstract]:Aim: to investigate the effects of erythropoietin (EPO) on the production of (eryptosis) and reactive oxygen species (Ros) in erythrocyte induced by hydrogen peroxide (H_2O_2), and to explore its possible mechanism. Methods: 1% healthy human erythrocyte suspension was incubated in the following three groups in vitro: control group (group C, PBS solution) and group H _ 2O _ 2 (H group, PBS solution with 100 渭 mol/L H_2O_2 final concentration) and EPO group (E group). The medium was PBS solution with final concentration of 100 渭 mol / L H_2O_2 and final concentration of 2 脳 10~4U/L. After incubation for 24 h and 60 h, red blood cells were collected for detection. The decay rate of red blood cells, the concentration of ROS and calcium in red blood cells were detected by flow cytometry. Results: erythrocyte decay rate increased with the increase of incubation time in group C. At the same observation point, the ROS production of erythrocytes in group H was significantly higher than that in group C (P0.01). The ROS production of erythrocytes in group E was significantly lower than that in group H (P0.01). The ROS production of erythrocytes in group H was significantly higher than that in group C (P0.01). The ROS production of erythrocyte in group E was significantly higher than that in group C (P0.01). The ROS production of erythrocyte in group E was significantly lower than that in group H, and that in group H was significantly lower than that in group H. Group A was significantly lower (P0.05 or P0.01). Conclusion\%\
【作者单位】： 山东大学第二医院肾内科;
【基金】：山东大学第二医院种子基金资助项目(No.S2015010001) 山东省自然科学基金资助项目(No.ZR2014HM045)
【分类号】：R692.5
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