脑特异性血管生成抑制因子1在膀胱尿路上皮癌中的表达及临床相关性研究

发布时间：2018-09-02 05:44

【摘要】：目的:本研究旨在明确BAI-1基因在膀胱正常组织和膀胱尿路上皮癌组织中的表达情况及其对膀胱尿路上皮癌实体肿瘤新生血管的作用;分析BAI-1基因表达与临床各病理参数之间的相关性,探讨BAI-1基因在膀胱尿路上皮癌的发生、发展中的作用,以期为膀胱尿路上皮癌的诊治提供帮助。方法:采用逆转录-聚合酶链反应(Reverse transcription-polymerase chain reaction,RT-PCR)检测20例膀胱正常组织和20例膀胱尿路上皮癌组织的BAI-1 mRNA的含量。在免疫组织化学SP法检测BAI-1表达情况的方法中,肌层浸润性膀胱尿路上皮癌、非肌层浸润性膀癌和正常膀胱组织分别为93例、39例和30例,同时检测肿瘤相关巨噬细胞(TAMs,即CD68+)及微血管密度(MVD,即CD34+)在上述组织中的表达。应用蛋白质印迹法(Western Blot)检测膀胱正常组织、非肌层浸润性和肌层浸润性膀胱尿路上皮癌中各自BAI-1蛋白含量的多少。回顾性分析132例患者的BAI-1基因表达的高低与所纳入的膀胱尿路上皮癌患者的各临床及病理因素的关系。结果:1、BAI-1 mRNA在膀胱正常组织中的表达明显高于癌组织,其差异具有统计学意义(p0.05)。2、BAI-1蛋白在膀胱正常组织中的表达明显高于癌组织,其差异具有统计学意义(p0.05)。3、BAI-1在膀胱尿路上皮癌中的表达与TAMs(CD68+)和MVD(CD34+)的表达均成负相关,而TAMs和MVD在膀胱尿路上皮癌中的表达则呈现出正相关关系,各自差异均具有统计学意义(p0.05)。4、BAI-1在膀胱尿路上皮癌中的表达的高低与年龄、性别、肿瘤大小、数目、吸烟及肿瘤的转移无关(p0.05),但是与肿瘤的分期、分级有关,(p0.05)。BAI-1的低表达对膀胱尿路上皮癌患者的总生存和无复发生存具有显著影响(log-rank p0.05)。结论:1、BAI-1 mRNA及其BAI-1基因所表达的BAI-1蛋白在正常膀胱组织中表达阳性率比膀胱尿路上皮癌组织中表达的高;且BAI-1比到达的高低与肿瘤的分期分级有关。2、BAI-1基因低表达与TAMs(CD68+)的高表达和与膀胱尿路上皮癌的高分期、高分级和复发密切关联,提示BAI-1的低表达与TAMs(CD68+)的高表达的负相关在膀胱尿路上皮癌的发生、发展中起到一定的作用。
[Abstract]:Objective: to investigate the expression of BAI-1 gene in bladder normal tissues and bladder urothelial carcinoma tissues and its role in the neovascularization of solid tumors of bladder urothelial carcinoma. To analyze the relationship between the expression of BAI-1 gene and the clinicopathological parameters, and to explore the role of BAI-1 gene in the occurrence and development of bladder urothelial carcinoma, in order to provide help for the diagnosis and treatment of bladder urothelial carcinoma. Methods: reverse transcription-polymerase chain reaction (Reverse transcription-polymerase chain reaction,RT-PCR) was used to detect the content of BAI-1 mRNA in 20 cases of normal bladder and 20 cases of bladder urothelial carcinoma. In the immunohistochemical SP method, the expression of BAI-1 was detected in 93 cases of bladder epithelial carcinoma, 30 cases of non-myometrial bladder carcinoma and 30 cases of normal bladder tissue, respectively, in which there were 93 cases of invasive bladder epithelial carcinoma, 30 cases of non-muscular invasive bladder carcinoma and 30 cases of normal bladder tissue. The expression of tumor associated macrophages (TAMs, CD68) and microvessel density (MVD, (CD34) was also detected. Western blot (Western Blot) was used to detect the content of BAI-1 protein in bladder normal tissue, non-myometrial infiltrating bladder and myometrial invasive bladder urothelial carcinoma. The relationship between the expression of BAI-1 gene and the clinical and pathological factors of bladder urothelial carcinoma was analyzed retrospectively. Results the expression of BAI-1 mRNA in normal bladder tissues was significantly higher than that in normal bladder tissues, and the difference was statistically significant (p0.05) .2the expression of BAI-1 protein in normal bladder tissues was significantly higher than that in cancer tissues. The difference was statistically significant (p0.05) .3BAI-1 expression in bladder urothelial carcinoma was negatively correlated with the expression of TAMs (CD68) and MVD (CD34), while the expression of TAMs and MVD in bladder urothelial carcinoma was positively correlated. The differences were statistically significant (p0.05). 4 the expression of BAI-1 in bladder urothelial carcinoma was not related to age, sex, tumor size, number, smoking and metastasis (p0.05). The low expression of (p0. 05). BAI-1 had a significant effect on the overall survival and recurrence free survival of patients with bladder urothelial carcinoma (log-rank p0. 05). Conclusion the positive rate of BAI-1 protein expression in normal bladder tissue is higher than that in bladder urothelial carcinoma tissue. The low expression of BAI-1 gene was associated with the high expression of TAMs (CD68) and the high grade, high grade and recurrence of bladder urothelial carcinoma. The results suggest that the low expression of BAI-1 and the high expression of TAMs (CD68) play a role in the occurrence and development of bladder urothelial carcinoma.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.14

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