螺内酯对高果糖诱发肾损伤的保护作用

发布时间：2018-09-04 16:32

【摘要】：目的探讨醛固酮受体阻断剂螺内酯对高果糖引起的肾组织损伤的保护作用及机制。方法选取Wistar雄性大鼠,180~200 g,共饲养8周,随机分3组(n=8):(1)对照组,给予正常大鼠饲料及蒸馏水;(2)高果糖(HF)组,给予正常饲料及高果糖水(10%,w/v);(3)螺内酯组,高果糖水给予4周后,加入螺内酯50 mg/(kg·d)(管饲法),持续4周。检测各组大鼠体质量、空腹血糖、血清胰岛素水平以及对各组大鼠进行组织学检测。结果高果糖可引起大鼠体质量、空腹血糖及动脉血压升高,血清胰岛素水平升高,另外高果糖可导致肾小球硬化、肥大,肾小管肿胀坏死,肾间质细胞外基质沉积,而螺内酯治疗4周可显著逆转上述各项指标,改善肾组织结构损伤。结论螺内酯改善高果糖引起的肾损伤可能与其抑制水钠潴留以及阻断醛固酮受体活化引起的胰岛素抵抗作用有关。
[Abstract]:Objective to investigate the protective effect and mechanism of aldosterone receptor blocker spironolactone on renal tissue injury induced by high fructose. Methods male Wistar rats were randomly divided into 3 groups: control group (n = 8): (1), normal rat diet and distilled water, (2) high fructose (HF) group, normal diet and high fructose water (10 wr / v); (3) spironolactone group, 4 weeks after high fructose water administration. Add spironolactone 50 mg/ (kg d) (tube feeding) for 4 weeks. Body mass, fasting blood glucose, serum insulin level and histology of each group were detected. Results High fructose could increase the body mass, fasting blood glucose, arterial blood pressure, serum insulin level, glomerulosclerosis, hypertrophy, tubule swelling and necrosis, and the deposition of extracellular matrix in renal interstitial cells. Spironolactone treatment for 4 weeks could significantly reverse the above indexes and improve renal tissue structure injury. Conclusion the effect of spironolactone on renal injury induced by high fructose may be related to the inhibition of sodium and water retention and the blocking of insulin resistance induced by the activation of aldosterone receptor.
【作者单位】：广东药科大学基础学院;广东药科大学公卫学院预防医学系;
【分类号】：R692

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