甘草次酸调节BLM的转录及PC3细胞的增殖凋亡和迁移侵袭

发布时间：2018-09-12 09:55

【摘要】：[目的]探究甘草次酸(GA)影响前列腺癌(PC3)细胞BLM基因的转录以及细胞的增殖、迁移、侵袭和凋亡的情况。[方法]MTT法检测细胞增殖能力并筛选适宜的药物浓度,荧光定量PCR检测BLM基因的表达量,划痕法检测细胞迁移,Transwell小室检测细胞侵袭,流式细胞仪检测细胞凋亡。[结果]甘草次酸浓度在0.12 mol/L~0.36 mol/L范围时,随着浓度增大,细胞的增殖能力逐渐下降,呈现药物浓度梯度依赖性;当甘草次酸浓度为0.2 mol/L时,PC3细胞中BLM的mRNA的表达量仅有对照组的0.2倍;甘草次酸浓度在0.10 mol/L以上时,细胞的侵袭和迁移能力均显著减小;流式结果显示甘草次酸浓度在0.10 mol/L时,细胞凋亡率为45.25±0.5**,差异极显著(P0.01)。[结论]甘草次酸浓度为0.15 mol/L以上时,能够下调PC3细胞中BLM基因的转录量(P0.01),在0.12 mol/L~0.36 mol/L范围时,对PC3细胞的增殖、侵袭、迁移具有抑制作用,对PC3细胞凋亡具有促进作用。
[Abstract]:[objective] to investigate the effects of glycyrrhetinic acid (GA) on the transcription of BLM gene and cell proliferation, migration, invasion and apoptosis in prostate cancer (PC3) cells. [methods] MTT assay was used to detect the cell proliferation ability and the appropriate drug concentration was screened, the expression of BLM gene was detected by fluorescence quantitative PCR, the cell invasion was detected by scratch assay, and apoptosis was detected by flow cytometry. [results] when the concentration of glycyrrhetinic acid was in the range of 0.12 mol/L~0.36 mol/L, the proliferation ability of the cells decreased gradually with the increase of the concentration, which showed a gradient dependence on the concentration of glycyrrhetinic acid. When the concentration of glycyrrhetinic acid was 0. 2 mol/L, the expression of BLM mRNA in PC3 cells was only 0. 2 times of that in the control group, the invasion and migration ability of the cells decreased significantly when the concentration of glycyrrhetinic acid was above 0. 10 mol/L, and the flow rate showed that the concentration of glycyrrhetinic acid was 0. 10 mol/L. The rate of apoptosis was 45.25 卤0.5, and the difference was very significant (P0.01). [conclusion] when the concentration of glycyrrhetinic acid is above 0.15 mol/L, the transcription of BLM gene in PC3 cells can be down-regulated (P0.01). At 0.12 mol/L~0.36 mol/L, it can inhibit the proliferation, invasion and migration of PC3 cells and promote the apoptosis of PC3 cells.
【作者单位】：高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州省动物遗传育种与繁殖重点实验室;贵州大学生命科学学院;
【基金】：国家自然科学基金项目(“RecQ解旋酶在前列腺癌细胞中的表达及作为抗癌药物靶标的研究”,No.31361406)
【分类号】：R737.25

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