维生素E对小鼠体内精子品质及精子DNA的保护作用

发布时间：2018-10-09 14:39

【摘要】：旨在研究维生素E对小鼠精子DNA的保护作用及对精液中抗氧化物酶活性的影响。以小鼠为动物模型,选日龄、大小相同的健康雄性小白鼠50只,随机分成5组,在试验组小鼠的每千克日粮中分别添加4、8、12、16g的维生素E,对照组添加量为0,饲喂30d后,通过颈椎脱臼处死小鼠,解剖后通过附睾和曲精细管获得小鼠精液。通过单细胞凝胶电泳及相关检测试剂盒检测分析各组间精液DNA的完整性、精子活力、密度、畸形率、精浆中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)、谷胱甘肽还原酶(GR)和过氧化氢酶(CAT)的活性。结果表明:每千克日粮中维生素E的添加量为12g时,0级精子百分率与对照组相比差异显著(P0.05);添加量为8~16g时,精子活力与对照组相比差异显著(P0.05);GR和SOD酶的活性与对照组相比差异显著(P0.05)。每千克日粮中维生素E添加量为12~16g时,CAT酶的活性与对照组相比差异显著(P0.05)。综上表明,在饲粮中添加维生素E浓度为12g·kg-1时可以明显提高小鼠精液中CAT、GR、GSH和SOD酶的活性及精子DNA的完整性。
[Abstract]:The aim of this study was to study the protective effect of vitamin E on DNA of mouse spermatozoa and the effect of vitamin E on the activity of antioxidant enzymes in semen. Using mice as animal model, 50 healthy male mice of the same size and day were randomly divided into 5 groups. Each kilogram of vitamin E was added to the diet of mice in the experimental group, and the control group was fed with 0 for 30 days. Mice were killed by dislocation of cervical vertebrae and semen was obtained from epididymis and convoluted fine tube after dissection. The integrity, sperm motility, density and abnormal rate of semen DNA were analyzed by single cell gel electrophoresis (SCGE) and correlation detection kit. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH), glutathione reductase (GR) and catalase (CAT) in seminal plasma. The results showed that the percentage of spermatozoa of grade 0 was significantly different from that of the control group when vitamin E supplementation was 12 g per kg diet (P0.05), and 816 g of vitamin E was added to the diet. Sperm motility was significantly different from the control group (P0.05). The activity of gr and SOD was significantly different from that of the control group (P0.05). The activity of catalase was significantly different from that of the control group when the vitamin E supplementation was 12g / kg (P0.05). The results showed that the activities of CAT,GR,GSH and SOD enzymes and the integrity of sperm DNA were significantly increased when vitamin E concentration was 12g kg-1 in diet.
【作者单位】：西北农林科技大学动物科技学院;渭南职业技术学院渭南市农产品食品检验检测研究中心;
【基金】：“十二五”国家863计划(2011AA100307-02) 国家自然科学基金(31272411) 新疆建设兵团重大科技计划(2014AA001-1) “十二五”国家转基因育种重大专项(2014ZX08007-002)
【分类号】：R698.2

【参考文献】