前列腺癌中Sp1通过PKM2途径促进细胞增殖代谢

发布时间：2018-10-14 09:52

【摘要】：目的:确定前列腺癌中Sp1通过PKM2途径对前列腺癌细胞的增殖和代谢的作用。方法:前列腺癌细胞株DU145与PC3体外转染Sp1 si RNA与阴性对照无意义核苷酸序列(NC组),采用葡萄糖检测试剂盒与乳酸检测试剂盒检测转染后有氧糖酵解变化;CCK-8实验检测转染后的细胞增殖;q RT-PCR检测转染后PKM2表达;Western Blotting检测转染后Sp1与PKM2表达。结果:DU145与PC3转染Sp1 si RNA后与NC组比较,剩余葡萄糖显著偏高,乳酸产生显著下降;CCK8实验表明抑制Sp1后能显著抑制前列腺癌细胞的增殖能力;q RT-PCR实验显示抑制Sp1后明显抑制PKM2的表达;Western Blotting显示转染的Sp1 si RNA对Sp1具有较高的沉默效率,且PKM2的表达也降低。结论:Sp1在前列腺癌细胞株DU145与PC3中可通过直接作用于PKM2促进细胞代谢。
[Abstract]:Aim: to determine the effect of Sp1 on the proliferation and metabolism of prostate cancer cells through PKM2 pathway. Methods: prostate cancer cell line DU145 and PC3 were transfected with Sp1 si RNA and negative control nucleotide sequence in vitro (NC group), glucose detection kit and lactic acid assay kit were used to detect the changes of aerobic glycolysis after transfection; CCK-8 test was used to detect the changes of aerobic glycolysis after transfection. The cell proliferation after transfection and the expression of PKM2 and PKM2 after transfection were detected by Q RT-PCR and; Western Blotting respectively. Results: after transfection of Sp1 si RNA with DU145 and PC3, the residual glucose was significantly higher than that in NC group. CCK8 assay showed that inhibition of Sp1 could significantly inhibit the proliferation of prostate cancer cells, and Q RT-PCR assay showed that Sp1 inhibited the expression of PKM2 significantly,; Western Blotting showed that the transfected Sp1 si RNA had a higher silencing efficiency to Sp1. The expression of PKM2 was also decreased. Conclusion: Sp1 can promote cell metabolism by directly acting on PKM2 in prostate cancer cell line DU145 and PC3.
【作者单位】：东南大学附属徐州市中心医院泌尿外科;东南大学附属中大医院泌尿外科;东南大学医学院外科中心实验室;
【基金】：国家自然基金(81370849、81300472、81272557、81202034) 临床医学科技专项——新型临床诊疗技术攻关基金(BL2013032) 教育部博士点基金(20120092120071) 江苏省自然科学基金(BK2012336、BK2012647) 南京市科技发展基金(201201053) 江苏六大人才高峰基金(WSW-067) 江苏省普通高校研究生科研创新计划基金(CXZZ13_0133) 中央高校基本科研业务费专项基金资助项目
【分类号】：R737.25

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