PIM家族激酶在前列腺癌中的表达及意义
发布时间：2018-10-14 11:31
【摘要】：目的：①系统性研究PIM家族激酶(PIM-1/-2/-3)在前列腺癌与良性前列腺增生组织及前列腺细胞系中的表达情况。②明确PIM激酶在前列腺癌中的表达水平与其临床相关性。③探讨PIM激酶是否通过其激酶结构域在前列腺癌的发生发展中发挥作用。 方法：收集2000年1月到2012年6月在天津医科大学第二医院经病理诊断确诊为前列腺癌的160例患者的术中癌组织和100例良性前列腺增生组织标本,所有前列腺癌患者术前均未接受内分泌治疗以及放化疗。培养前列腺癌细胞系LNCaP及PC3,正常前列腺上皮细胞系RWPE-1.采用组织总RNA提取、cDNA合成、RT-qPCR以及Western Blot等技术,分析PIM激酶在前列腺癌与良性前列腺增生组织及前列腺细胞系中的表达水平,并研究其表达水平与前列腺癌临床病理特征以及前列腺癌患者PSA生化复发的相关性。 结果：①PIM-1/-2/-3在前列腺癌组织中的mRNA表达水平均显著高于良性前列腺增生组织(P0.05)；②PIM-1/-2/-3在前列腺癌细胞系中蛋白表达水平均显著高于正常前列腺上皮细胞(P0.05)；③PIM-1/-2/-3的高表达与血清前列腺特异性抗原(PSA),较高的Gleason评分,高级别肿瘤病理分期(T3),淋巴结浸润及神经周围浸润有关(P0.05)；④PIM-1/-2/-3的高表达与患者的年龄,手术切缘阳性以及精囊侵犯没有必然的联系(P0.05)；⑤PIM-1/-2/-3的高表达与前列腺癌患者PSA生化复发有关,具有统计学意义(P0.05)。 结论：①PIM家族激酶所有三个成员在前列腺癌中的表达均显著高于良性前列腺增生组织以及正常前列腺上皮细胞,表明其共同的组织分布特性；②PIM家族激酶的高表达均与前列腺癌的发生和进展具有相关性,表明其存在相近的生理特性；③PIM激酶在前列腺癌中可能通过其激酶结构域发挥作用,PIM蛋白激酶结构域可能成为研究前列腺癌发病机制的一个重要突破点,并成为治疗前列腺癌的一个潜在靶点,但还有待于进一步研究。
[Abstract]:Objective: 1 to systematically study the expression of PIM family kinase (PIM-1/-2/-3) in prostate cancer and benign prostatic hyperplasia (BPH) tissues and prostatic cell lines. To investigate whether PIM kinase plays a role in the development of prostate cancer through its kinase domain. Methods: from January 2000 to June 2012, 160 cases of prostate cancer and 100 cases of benign prostatic hyperplasia were collected from the second Hospital of Tianjin Medical University. All patients with prostate cancer were not treated with endocrine therapy or radiotherapy and chemotherapy before operation. Culture of Prostate Cancer Cell Line LNCaP and PC3, normal Prostate epithelial Cell Line RWPE-1. Total RNA extraction, cDNA synthesis, RT-qPCR and Western Blot were used to analyze the expression of PIM kinase in prostate cancer and benign prostatic hyperplasia (BPH) and prostatic cell line. To study the correlation between the expression level and clinicopathological features of prostate cancer and the biochemical recurrence of PSA in patients with prostate cancer. Results: the mRNA expression of 1PIM-1/-2/-3 in prostate cancer was significantly higher than that in benign prostatic hyperplasia (P0.05), and the expression of 2PIM-1/-2/-3 in prostate cancer cell line was significantly higher than that in normal prostatic epithelial cell (P0.05). The high expression of 3PIM-1/-2/-3 was related to the higher Gleason score of serum prostate specific antigen (PSA), the pathological stage of high grade tumor (T3), lymph node invasion and peripheral nerve infiltration (P0.05), the high expression of 4PIM-1/-2/-3 was associated with the age of the patients. The positive margin of surgical incision and seminal vesicle invasion were not associated (P0.05); the high expression of 5PIM-1/-2/-3 was related to the recurrence of PSA in prostate cancer patients with statistical significance (P0.05). Conclusion: the expression of all three members of 1PIM family kinase in prostate cancer is significantly higher than that in benign prostatic hyperplasia and normal prostatic epithelial cells, indicating their common tissue distribution characteristics. The high expression of 2PIM family kinases was associated with the occurrence and progression of prostate cancer, indicating that there were similar physiological characteristics. 3PIM kinase may play a role in prostate cancer through its kinase domain. PIM protein kinase domain may become an important breakthrough point in studying the pathogenesis of prostate cancer and a potential target for the treatment of prostate cancer. But it still needs further study.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25
本文编号：2270329
[Abstract]:Objective: 1 to systematically study the expression of PIM family kinase (PIM-1/-2/-3) in prostate cancer and benign prostatic hyperplasia (BPH) tissues and prostatic cell lines. To investigate whether PIM kinase plays a role in the development of prostate cancer through its kinase domain. Methods: from January 2000 to June 2012, 160 cases of prostate cancer and 100 cases of benign prostatic hyperplasia were collected from the second Hospital of Tianjin Medical University. All patients with prostate cancer were not treated with endocrine therapy or radiotherapy and chemotherapy before operation. Culture of Prostate Cancer Cell Line LNCaP and PC3, normal Prostate epithelial Cell Line RWPE-1. Total RNA extraction, cDNA synthesis, RT-qPCR and Western Blot were used to analyze the expression of PIM kinase in prostate cancer and benign prostatic hyperplasia (BPH) and prostatic cell line. To study the correlation between the expression level and clinicopathological features of prostate cancer and the biochemical recurrence of PSA in patients with prostate cancer. Results: the mRNA expression of 1PIM-1/-2/-3 in prostate cancer was significantly higher than that in benign prostatic hyperplasia (P0.05), and the expression of 2PIM-1/-2/-3 in prostate cancer cell line was significantly higher than that in normal prostatic epithelial cell (P0.05). The high expression of 3PIM-1/-2/-3 was related to the higher Gleason score of serum prostate specific antigen (PSA), the pathological stage of high grade tumor (T3), lymph node invasion and peripheral nerve infiltration (P0.05), the high expression of 4PIM-1/-2/-3 was associated with the age of the patients. The positive margin of surgical incision and seminal vesicle invasion were not associated (P0.05); the high expression of 5PIM-1/-2/-3 was related to the recurrence of PSA in prostate cancer patients with statistical significance (P0.05). Conclusion: the expression of all three members of 1PIM family kinase in prostate cancer is significantly higher than that in benign prostatic hyperplasia and normal prostatic epithelial cells, indicating their common tissue distribution characteristics. The high expression of 2PIM family kinases was associated with the occurrence and progression of prostate cancer, indicating that there were similar physiological characteristics. 3PIM kinase may play a role in prostate cancer through its kinase domain. PIM protein kinase domain may become an important breakthrough point in studying the pathogenesis of prostate cancer and a potential target for the treatment of prostate cancer. But it still needs further study.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25
【参考文献】
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1 韩苏军;张思维;陈万青;李长岭;;中国前列腺癌发病现状和流行趋势分析[J];临床肿瘤学杂志;2013年04期
，本文编号：2270329
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