根皮素引起前列腺癌LNCaP细胞发生凋亡的机制研究

发布时间：2018-10-15 14:42

【摘要】：根皮素是存在于苹果、梨等水果及多种蔬菜汁液中的天然活性物质,具有抗肿瘤活性,能够有效地诱导细胞凋亡、抑制细胞增殖.本研究初步探讨了根皮素诱导前列腺癌LNCaP细胞的凋亡和相关分子机制.通过形态学观察、噻唑蓝(MTT)比色实验、CCK-8实验测定前列腺癌细胞活性;采用DAPI染色法测定细胞核凝缩破裂;运用Annexin V-FITC/PI双染流式细胞术测定前列腺癌细胞的凋亡率;再通过PI单染流式细胞术测定前列腺癌细胞的周期变化;以及采用Western blot检测PI3K/AKT信号通路及其下游细胞凋亡相关蛋白和细胞周期相关蛋白的表达.实验结果表明:根皮素诱导前列腺癌LNCaP细胞周期阻滞在G2/M期,并呈浓度依赖性降低细胞活性、增加细胞核凝缩破裂,显著提高前列腺癌细胞的凋亡率.在分子机制上,根皮素呈现浓度依赖性下调PI3K/AKT的活性,从而影响细胞凋亡相关蛋白和细胞周期相关蛋白的表达.因此,根皮素对前列腺癌细胞的生长有显著的抑制作用,对治疗前列腺癌具有潜在的临床应用价值.
[Abstract]:Phyllostatin is a natural active substance in fruits and vegetables such as apple pear and so on. It has anti-tumor activity and can effectively induce cell apoptosis and inhibit cell proliferation. In this study, the apoptosis of prostate cancer LNCaP cells induced by phylloderin and its related molecular mechanisms were investigated. Morphological observation, (MTT) colorimetric assay, CCK-8 assay, DAPI staining, Annexin V-FITC/PI double staining flow cytometry were used to determine the apoptosis rate of prostate cancer cells. The cell cycle changes of prostate cancer cells were detected by PI single staining flow cytometry, and the expression of apoptosis-related proteins and cell cycle associated proteins in PI3K/AKT signaling pathway and its downstream were detected by Western blot. The results showed that LNCaP cell cycle arrest in G _ 2 / M phase was induced by phylloderin in a concentration-dependent manner, which decreased cell activity, increased nuclear condensation and increased apoptosis rate of prostate cancer cells. In molecular mechanism, hirsutin down-regulated the activity of PI3K/AKT in a dose-dependent manner, thus affecting the expression of apoptosis-related proteins and cell cycle related proteins. Therefore, the growth of prostate cancer cells was significantly inhibited by phylloderin, which had a potential clinical value in the treatment of prostate cancer.
【作者单位】：南京师范大学生命科学学院江苏省分子与医学生物技术重点实验室;
【基金】：国家自然科学基金(81272850、81472415)
【分类号】：R737.25

【参考文献】