伊马替尼对尿毒症心肌病大鼠心肌纤维化的保护作用

发布时间：2018-10-21 18:28

【摘要】：目的观察伊马替尼通过调控PDGFRα的表达改善尿毒症大鼠心肌纤维化的作用。方法将72只大鼠分为Sham组,5/6组和5/6+I组,5/6组建立大鼠尿毒症模型,5/6+I组建模后行伊马替尼灌胃,Sham组仅行肾脏游离。所有大鼠于术后8周行心脏病理染色;实时定量荧光PCR、免疫组织化学测定心脏血小板衍生生长因子受体α(PDGFRα)mRNA及蛋白表达。结果 5/6组和5/6+I组大鼠尿蛋白、Scr、BUN均较Sham组明显增加(P0.01).Sham组心肌病理评分显著低于5/6组(P0.01),而5/6+I组显著低于5/6组(P0.01);5/6组心肌间质胶原容积分数值明显高于Sham组(P0.01);5/6+I组高于Sham组(P0.05),但与5/6组比较,明显下降(P0.05);5/6组和5/6+I组的PDGFRαmRNA和蛋白表达均显著高于Sham组(均P0.01);而5/6+I组明显低于5/6组(P0.05)。结论伊马替尼可以通过抑制PDGFRα表达减少尿毒症大鼠心脏病理损伤及纤维化的程度。
[Abstract]:Aim to observe the effect of imatinib on myocardial fibrosis in uremic rats by regulating the expression of PDGFR 伪. Methods 72 rats were divided into Sham group, 5 / 6 group and 5 / 6 I group. The expression of platelet derived growth factor receptor 伪 (PDGFR 伪) mRNA and protein was detected by real-time quantitative PCR, immunohistochemistry. Results urinary protein and Scr,BUN of rats in 5 / 6 and 5 / 6 I groups were significantly higher than those in Sham group (P0.01). Sham group was significantly lower than that of 5 / 6 group (P0.01), and 5 / 6 I group was significantly lower than 5 / 6 group (P0.01); 5 / 6 group was significantly higher than Sham group (P0.01); 5 / 6 I group was significantly higher than that of Sham group (P0.01); group 5 / 6 I group was significantly lower than that of Sham group (P0.01); group 5 / 6 I group was significantly lower than 5 / 6 group (P0.01). Higher than Sham group (P0.05), but compared with 5 / 6 group, The expression of PDGFR 伪 mRNA and protein in 5 / 6 group and 5 / 6 I group was significantly higher than that in Sham group (P0.01), while the expression of PDGFR 伪 mRNA and protein in 5 / 6 I group was significantly lower than that in 5 / 6 group (P0.05). Conclusion imatinib can reduce the degree of cardiac injury and fibrosis in uremic rats by inhibiting the expression of PDGFR 伪.
【作者单位】：泸州医学院附属医院肾病内科;泸州医学院附属中医医院中西医结合研究中心;泸州医学院中西医结合学院;四川大学生物治疗国家重点实验室;
【基金】：国家自然科学基金项目(81170667) 四川省科技厅项目(2011JTD0014、2013CDLZ-S20、14JC01503-LH48) 泸州市科技厅项目[2015-R-55(8/16)]
【分类号】：R542.2;R692.5

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