炎症因子在非特异性输尿管炎表达的相关研究

发布时间：2018-10-26 14:52

【摘要】：目的 1、非特异性输尿管炎的临床报道较少,本文通过对30例非特异性输尿管炎临床相关资料的回顾性分析,探讨非特异性输尿管炎的临床特点与诊断治疗要点。 2、非特异性输尿管炎的病因机制迄今不清,本文通过检测多种炎症因子在非特异性输尿管炎的表达情况,探讨炎症因子在非特异性输尿管炎发病机制中的作用。材料与方法收集天津医科大学第二医院、天津医科大学静海临床学院临床资料完整、行手术切除、病理证实的非特异性输尿管炎病理标本30例,收集10例肾癌根治术及肾盂癌根治术的输尿管标本做对照。两组性别、年龄、输尿管取材部位相匹配。 1、对30例非特异性输尿管炎患者的临床资料进行回顾性分析,总结特异性输尿管炎的临床特点。 2、采用免疫组化法检测输尿管粘膜标本中TGF-β、IL-1、IL-6、IL-8、IL-17、IL-23的表达情况。运用图像分析技术计数切片中阳性细胞数,取光学显微镜下高倍视野(×400)阳性细胞表达强度作为计数标准。计量资料应用t检验分析,计数资料应用x2检验和分类变量的等级相关分析,P0.05认为有统计学差异。结果 1、非特异性输尿管炎是一种病因不十分清楚的节段性非特异性输尿管炎症,好发于输尿管中下段,上段相对少见,以女性下尿路易感人群为多见。病理特点为输尿管管壁呈现不同程度的炎性细胞浸润。该病缺乏特异性临床表现,影像学及输尿管镜检查为主要确诊手段。治疗应该根据患侧肾功能状况以及输尿管病变的程度、部位和范围而确定。 2、在非特异性输尿管炎和对照组输尿管中TGF-β、IL-1、IL-6、IL-8、IL-17、IL-23的表达有统计学差异(P0.05),在非特异性输尿管炎中表达强度高于对照组输尿管。结论 1、非特异性输尿管炎临床表现缺乏特异性,诊断主要根据影像学及病理特点,治疗目的为解除输尿管梗阻和保护肾功能。 2、非特异性输尿管炎TGF-β、IL-1、IL-6、IL-8、IL-17、IL-23的表达强度明显高于对照组输尿管,提示以上六种细胞因子在非特异性输尿管炎疾病的发生、发展中具有重要的作用。为今后非特异性输尿管炎疾病的病因学研究提供了理论基础,同时开辟了以细胞因子为切入点治疗非特异性输尿管炎疾病的新途径。
[Abstract]:Objective 1. There are few clinical reports on nonspecific ureteritis. The clinical features and diagnosis and treatment of nonspecific ureteritis were discussed by retrospective analysis of 30 cases of nonspecific ureteritis. 2. The etiological mechanism of nonspecific ureteritis is unclear. The expression of various inflammatory factors in nonspecific ureteritis was detected to explore the role of inflammatory factors in the pathogenesis of nonspecific ureteritis. Materials and methods 30 cases of nonspecific ureteritis were collected from the second Hospital of Tianjin Medical University and Jinghai Hospital of Tianjin Medical University. The ureteral specimens of 10 cases of radical renal carcinoma and radical resection of renal pelvis carcinoma were collected and compared. The sex, age and site of ureter were matched. 1. The clinical data of 30 patients with nonspecific ureteritis were retrospectively analyzed and the clinical features of specific ureteritis were summarized. 2. Immunohistochemical method was used to detect the expression of TGF- 尾 and IL-1,IL-6,IL-8,IL-17,IL-23 in ureteral mucosa. The number of positive cells in sections was counted by image analysis technique, and the intensity of positive cells in high power field of vision (脳 400) under optical microscope was taken as the counting standard. The measurement data were analyzed by t test, the count data by x2 test and the rank correlation analysis of classified variables. Results 1. Nonspecific ureteritis is a segmental nonspecific ureteritis with unclear etiology. It is more common in the middle and lower ureteral segment than in the upper ureteral segment, especially in the female lower urinary tract susceptible population. Pathological features of the ureteral wall showed different degrees of inflammatory cell infiltration. Lack of specific clinical manifestations, imaging and ureteroscopy as the main means of diagnosis. Treatment should be determined according to the renal function of the affected side and the extent, location and extent of ureteral lesions. (2) the expression of TGF- 尾 and IL-1,IL-6,IL-8,IL-17,IL-23 in ureter of nonspecific ureteritis was significantly different from that in control group (P0.05). In nonspecific ureteritis, the expression level was higher than that in the control group. Conclusion 1. The clinical manifestation of nonspecific ureteritis is lack of specificity. The diagnosis is mainly based on the imaging and pathological features. The purpose of treatment is to relieve ureteral obstruction and protect renal function. 2. The expression of TGF- 尾 and IL-1,IL-6,IL-8,IL-17,IL-23 in nonspecific ureteritis was significantly higher than that in control group, suggesting that the above six cytokines might occur in nonspecific ureteritis. Development plays an important role. It provides a theoretical basis for the etiological study of nonspecific ureteritis in the future and opens up a new approach to treat nonspecific ureteritis disease with cytokines as the cut-in point.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R693.3

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