重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响

发布时间：2018-11-01 15:13

【摘要】：目的:研究重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响。方法:将肾母细胞瘤细胞分别与高、中、低浓度(1×10~9、1×10~8、1×10~7VP/m L)的重组人p53腺病毒注射液共同孵育20 h,以不加注射液的细胞为空白对照。检测细胞增殖、周期、凋亡及相关基因p21、Bax蛋白表达,检测细胞中自噬相关基因LC-3、Atg7、Atg12表达和自噬体数量。结果:高、中、低浓度重组人p53腺病毒注射液对肾母细胞瘤细胞的增殖抑制率分别为(42.86±3.18)%、(33.64±7.25)%、(16.26±9.07)%,细胞凋亡率分别为(53.85±9.36)%、(37.35±9.64)%、(23.64±10.65)%。与空白对照比较,高、中、低浓度药物作用下G_0/G_1期细胞均增加,其中高、中浓度药物效果较明显(P0.05或P0.01);高浓度下细胞中p21、Bax蛋白和LC-3、Atg7、Atg12基因表达增强,自噬体数量增加(P0.01);中浓度下细胞中Bax蛋白表达增强(P0.05),其余指标无明显变化(P0.05)。结论:重组人p53腺病毒注射液可抑制肾母细胞瘤细胞增殖,诱导细胞凋亡和自噬。
[Abstract]:Aim: to study the effects of recombinant human p53 adenovirus injection on proliferation, apoptosis and autophagy of nephroblastoma cells. Methods: nephroblastoma cells were incubated with recombinant human p53 adenovirus injection at high, medium and low concentrations (1 脳 10 ~ (9) and 1 脳 10 ~ (8) 10~7VP/m L for 20 h, respectively. The cells without injection were used as blank control. The cell proliferation, cell cycle, apoptosis and the expression of p21 Bax-Bax protein were detected. The expression of autophagy associated gene LC-3,Atg7,Atg12 and the number of autophagy were detected. Results: the inhibitory rates of high, medium and low concentrations of recombinant human p53 adenovirus injection on the proliferation of nephroblastoma cells were (42.86 卤3.18)%, (33.64 卤7.25)%, (16.26 卤9.07)%, respectively. The apoptosis rates were (53.85 卤9.36)%, (37.35 卤9.64)%, (23.64 卤10.65)%, respectively. Compared with blank control, high, medium and low concentrations of drugs increased in G_0/G_1 phase cells, among which the effect of middle concentration of drugs was more obvious (P0.05 or P0.01). At high concentration, the expression of p21 Bax protein and LC-3,Atg7,Atg12 gene increased and the number of autophagy increased (P0.01), while the expression of Bax protein increased at medium concentration (P0.05), while the other indexes did not change significantly (P0.05). Conclusion: recombinant human p53 adenovirus injection can inhibit the proliferation of nephroblastoma cells and induce apoptosis and autophagy.
【作者单位】：河北医科大学第二医院小儿外科;
【基金】：河北省2013年医学科学研究计划基金项目(No.20130151)
【分类号】：R737.11

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