尿脱落细胞染色体畸变与膀胱癌组织学关系的研究

发布时间：2018-11-10 19:35

【摘要】：目的:探讨荧光原位杂交(FISH)检测尿脱落细胞常见染色体畸变与膀胱癌病理分级、分期的关系。方法:99例患者尿液标本采用FISH技术检测9p21位点及3、7、17号染色体的畸变,并收集术后肿瘤分级、分期信息。结果:(1)3、17号染色体的畸变率在不同分期、分级间差异均有统计学意义(P0.05),7号染色体的畸变率在不同分级间差异有统计学意义(P0.05),在不同病理分期间差异无统计学意义(P0.05)。9p21位点的畸变率在不同病理分期、分级间差异均无统计学意义(P0.05)。(2)病理分级、分期越高,3、7、17号染色体扩增倍数越高。分级、分期越低,9p21出现缺失几率越大;分级、分期升高,9p21出现扩增几率越大。结论:4种染色体畸变尤其是扩增与膀胱癌恶性度、肌层浸润密切相关,多倍体数目可能成为膀胱癌预后预测的一个生物学指标。
[Abstract]:Objective: to investigate the relationship between common chromosome aberration of exfoliated urine cells and pathological grade and stage of bladder cancer by fluorescence in situ hybridization (FISH). Methods: FISH technique was used to detect the aberration of 9p21 locus and chromosome 37 and 17 in 99 urine samples. The information of tumor grading and staging after operation was collected. Results: (1) the aberration rate of chromosome 3,17 was significantly different in different stages and grades (P0.05), and the aberration rate of chromosome 7 was significantly different among different grades (P0.05). There was no significant difference between different pathological stages (P0.05). The aberration rate of 9p21 locus was not statistically significant in different pathological stages (P0.05). (2). The amplification multiple of chromosome 17 was higher. The lower the stage, the greater the probability of 9p21 deletion, and the higher the stage, the greater the probability of 9p21 amplification. Conclusion: the four chromosomal aberrations, especially the amplification, are closely related to the malignancy and myometrial infiltration of bladder cancer, and the number of polyploids may be a biological index to predict the prognosis of bladder cancer.
【作者单位】：柳州市人民医院泌尿外科;广州军区广州总医院泌尿外科全军泌尿外科中心;
【基金】：国家自然科学基金面上项目(编号:No.81372744);国家自然科学基金青年科学基金资助项目(编号:81001142)
【分类号】：R737.14

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