IGF1和IGFBP3基因变异与中国人群肾癌易感性的相关性

发布时间：2018-11-13 19:29

【摘要】：目的:胰岛素样生长因子(insulin-like growth factor 1,IGF1)和胰岛素样生长因子结合蛋白-3(IGF binding protein 3,IGFBP3)在肾细胞癌的发生发展中起重要作用。本研究旨在探索位于IGF1和IGFBP3基因功能区具有潜在功能学的基因遗传多态性与中国人群肾癌易感性的关系。方法 :采用病例-对照研究方法,对1 027例肾癌患者和1 094例健康对照,应用Taq Man探针分型法对位于IGF1和IGFBP3基因功能区9个基因遗传多态性位点进行分型。控制混杂因素,应用非条件逻辑回归,评价基因多态性与肾癌易感性之间的关系。结果:在9个基因多态性位点中,通过校正后的多因素分析发现位于IGF1 3′-UTR区域的rs5742714多态性和中国人群肾癌发病风险明显相关。与携带IGF1 rs5742714 GG基因型的个体相比,IGF1 rs5742714 GC/CC基因型携带者的肾癌发病风险明显增高(OR=0.82;95%CI=0.68~0.98,P=0.002)。此外,IGF1 rs5742714 GC/CC基因型癌旁组织中IGF1 m RNA含量明显降低。结论:位于IGF1 3′-UTR区域的rs5742714多态性可能通过调控IGF1基因的表达而影响中国人群肾癌发病易感性。
[Abstract]:Objective: insulin like growth factor (insulin-like growth factor 1) and insulin-like growth factor binding protein 3 (IGF binding protein 3 (IGFBP3) play an important role in the development of renal cell carcinoma (RCC). The aim of this study was to explore the relationship between genetic polymorphisms with potential functional polymorphisms in the functional regions of IGF1 and IGFBP3 genes and susceptibility to renal cell carcinoma in Chinese population. Methods: a case-control study was carried out in 1 027 patients with renal cell carcinoma and 1 094 healthy controls. Nine genetic polymorphism loci in the functional regions of IGF1 and IGFBP3 were classified by Taq Man probe method. To evaluate the relationship between gene polymorphism and susceptibility to renal cell carcinoma (RCC) by controlling confounding factors and using non-conditional logistic regression. Results: among the 9 loci of gene polymorphisms, the rs5742714 polymorphism located in the IGF1 3'-UTR region was significantly correlated with the risk of renal cell carcinoma in Chinese population by adjusted multivariate analysis. Compared with individuals with IGF1 rs5742714 GG genotype, the risk of renal cell carcinoma in IGF1 rs5742714 GC/CC carriers was significantly higher (OR=0.82;95%CI=0.68~0.98,P=0.002). In addition, the content of IGF1 m RNA in paracancerous tissues of IGF1 rs5742714 GC/CC genotypes was significantly decreased. Conclusion: the rs5742714 polymorphism located in IGF1 3'-UTR region may influence the susceptibility of RCC in China by regulating the expression of IGF1 gene.
【作者单位】：吴江区第一人民医院泌尿外科;南京医科大学第一临床医学院;江阴市人民医院泌尿外科;南京医科大学第一附属医院泌尿外科;
【基金】：苏州市吴江区卫生局2014年度“科教兴卫”卫生科技项目(WWK201409) 江苏省高等学校大学生实践创新训练计划(201610312024Z,201410312017Z)
【分类号】：R737.11

【相似文献】