输精管结扎对大鼠睾丸网的影响:形态定量研究
发布时间：2018-11-17 14:20
【摘要】：目的:研究大鼠输精管结扎后睾丸网的组织学变化,以探讨输精管结扎致精子发生损害的机制。方法:12只10-11周龄的正常雄性(Sprague-Dawley,SD)大鼠,行单侧(随机选择左侧或者右侧)输精管结扎,结扎位置远离附睾尾和阴囊。术后37天,取大鼠双侧睾丸。沿垂直于睾丸长轴的方向,把每个睾丸都切成6片,每片切成2块,全部用甲基丙烯酸树脂包埋,制作切片,进行组织学观察,并运用体视学方法定量研究睾丸网的宽度和总体积。结果:与未结扎侧相比,12只大鼠结扎侧睾丸网的宽度显著增加了66.1%±29.4%(配对t检验,P0.05),但睾丸网的总体积没有变化。其中7只大鼠结扎侧睾丸的精子发生未见明显损害,但另5只大鼠见明显损害;单独看这两个亚组,结扎侧睾丸网的宽度都增加了64%-67%,尽管这没有统计学意义(P0.16)。结论:大鼠输精管结扎会导致睾丸网增宽,提示结扎后睾丸液可能淤积,生精小管内压可能增加,这支持输精管结扎对精子发生损害的压力介导机制。
[Abstract]:Aim: to study the histological changes of testicular reticulum after vasectomy in rats and to explore the mechanism of spermatogenesis induced by vas deferens ligation. Methods: 12 normal male (Sprague-Dawley,SD) rats aged 10-11 weeks were treated with unilateral (left or right) vasectomy, located far from the caudal epididymis and scrotum. After 37 days, bilateral testis were taken from rats. Along the direction perpendicular to the long axis of the testis, each testis was cut into 6 pieces, each of which was cut into 2 pieces, all of which were covered with methacrylic resin, and were made into sections and histologically observed. The width and total volume of testicular reticulum were quantitatively studied by stereological method. Results: compared with the unligated side, the width of testicular reticulum in 12 rats increased significantly by 66.1% 卤29.4% (pairing t test, P0.05), but the total volume of testicular reticulum did not change. There was no significant damage to spermatogenesis in 7 of them, but obvious damage was found in the other 5 rats. Looking at the two subgroups alone, the width of the ligated lateral testicular reticulum increased by 64-67, although there was no statistical significance (P 0. 16). Conclusion: ligation of vas deferens may lead to the enlargement of testicular reticulum, suggesting that testicular fluid may accumulate after ligation, and the pressure of seminiferous tubules may increase, which supports the pressure-mediated mechanism of vasectomy on spermatogenesis.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R699.8
本文编号：2338099
[Abstract]:Aim: to study the histological changes of testicular reticulum after vasectomy in rats and to explore the mechanism of spermatogenesis induced by vas deferens ligation. Methods: 12 normal male (Sprague-Dawley,SD) rats aged 10-11 weeks were treated with unilateral (left or right) vasectomy, located far from the caudal epididymis and scrotum. After 37 days, bilateral testis were taken from rats. Along the direction perpendicular to the long axis of the testis, each testis was cut into 6 pieces, each of which was cut into 2 pieces, all of which were covered with methacrylic resin, and were made into sections and histologically observed. The width and total volume of testicular reticulum were quantitatively studied by stereological method. Results: compared with the unligated side, the width of testicular reticulum in 12 rats increased significantly by 66.1% 卤29.4% (pairing t test, P0.05), but the total volume of testicular reticulum did not change. There was no significant damage to spermatogenesis in 7 of them, but obvious damage was found in the other 5 rats. Looking at the two subgroups alone, the width of the ligated lateral testicular reticulum increased by 64-67, although there was no statistical significance (P 0. 16). Conclusion: ligation of vas deferens may lead to the enlargement of testicular reticulum, suggesting that testicular fluid may accumulate after ligation, and the pressure of seminiferous tubules may increase, which supports the pressure-mediated mechanism of vasectomy on spermatogenesis.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R699.8
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