正常前列腺间质细胞对前列腺癌细胞糖代谢水平的影响

发布时间：2018-11-18 20:22

【摘要】：目的:探讨正常前列腺组织不同区带来源的前列腺间质细胞对前列腺癌细胞生长的影响及其作用机制。方法:以激素非依懒性前列腺癌细胞系DU145细胞为研究对象,取新鲜的正常前列腺外周带(PZ)和移行带(TZ)组织,提取间质细胞并体外培养。收集不同区带来源的间质细胞培养上清作为条件培养液培养DU145细胞,CCK8法测定肿瘤细胞的生长曲线,台盼蓝染色测定细胞数量及活力,细胞划痕实验测定细胞侵袭性,Western印迹法测定间质细胞对肿瘤细胞糖代谢关键酶的影响。结果:1PZ间质细胞的条件培养液能促进肿瘤细胞的生长,而TZ间质细胞的条件培养液抑制肿瘤细胞的生长;2PZ间质细胞的条件培养液明显增加肿瘤细胞糖代谢关键酶己糖激酶2(HK-2)、丙酮酸激酶2(PKM-2)、乳酸脱氢酶(LDHA)、丙酮酸脱氢酶(PDH)的表达,而TZ则抑制上述酶的表达。结论:前列腺不同来源的间质细胞对肿瘤细胞的生长影响不同,其机制可能与不同来源的间质细胞对糖酵解代谢的影响不同有关。
[Abstract]:Aim: to investigate the effects of prostatic stromal cells from different zones of normal prostate tissue on the growth of prostate cancer cells and its mechanism. Methods: the stromal cells of DU145 cell line were isolated and cultured in vitro. The fresh (PZ) and (TZ) tissues of the normal prostate were collected and cultured in vitro. Mesenchymal cell culture supernatants from different zones were collected as conditioned medium to culture DU145 cells. The growth curves of tumor cells were measured by CCK8 assay, the number and viability of tumor cells were determined by trypan blue staining, and the invasiveness of tumor cells was measured by cell scratch assay. The effects of interstitial cells on the key enzymes of glucose metabolism in tumor cells were determined by Western blot. Results: the conditioned medium of 1PZ stromal cells could promote the growth of tumor cells, while the conditioned medium of TZ stromal cells inhibited the growth of tumor cells. The expression of hexokinase 2 (HK-2), pyruvate kinase 2 (PKM-2) and lactate dehydrogenase (LDHA),) pyruvate dehydrogenase (PDH) were significantly increased in the conditioned medium of 2PZ mesenchymal cells. TZ inhibited the expression of these enzymes. Conclusion: the effects of stromal cells from different prostatic sources on the growth of tumor cells may be related to the different effects of stromal cells from different prostatic sources on glycolytic metabolism.
【作者单位】：上海交通大学医学院附属第九人民医院泌尿外科;
【基金】：国家自然科学基金(81172450;81202008) 上海市卫生局项目(20124Y160) 上海交通大学医学院科技基金(13XJ10019)~~
【分类号】：R737.25

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