普罗布考对肾脏缺血再灌注损伤大鼠的保护作用及机制
发布时间：2018-12-12 03:27
【摘要】：目的观察普罗布考对缺血再灌注大鼠肾脏氧化应激损伤的保护作用,同时探讨其作用机制。方法雄性SD大鼠30只,随机分为假手术组(S组)、缺血再灌注组(IR组)、普罗布考治疗组(P+IR组),每组10只。P+IR组大鼠每天用普罗布考(500mg/kg)灌胃,S组和IR组大鼠每天用等量温开水灌胃。1周后制备肾缺血再灌注大鼠模型:3组大鼠都摘除右肾,IR组和P+IR组用无创动脉夹夹闭左侧肾动脉,30min后松夹恢复血流再灌注。再灌注后S组和IR组每天温开水灌胃,P+IR组每天普罗布考灌胃,连续1周。1周后处死所有大鼠,留取血及肾组织标本,检测大鼠各项血液生化指标,观察肾组织病理学改变。结果与IR组相比,P+IR组血尿素氮(BUN)、血肌酐(SCr)、血脂水平明显降低(均P0.05),血清和肾组织中SOD活性明显上升、MDA含量明显下降(均P0.05),肾组织病理损伤减轻,肾指数明显改善(P0.05)。结论普罗布考能提高缺血再灌注损伤大鼠血及肾组织中SOD活性,改善肾功能,降低血脂水平,减轻肾组织病理损伤,发挥肾脏保护作用。
[Abstract]:Objective to observe the protective effect of Probucol on renal oxidative stress injury in ischemic reperfusion rats and to explore its mechanism. Methods Thirty male SD rats were randomly divided into three groups: sham operation group (S group), ischemia reperfusion group (IR group) and probucol treatment group (P IR group). 10. P IR rats in each group were treated with 500mg/kg daily. Rats in group S and group IR were perfused with the same amount of boiling water every day. 1 week later, the rat model of renal ischemia-reperfusion was established. The right kidney was excised in three groups, and the left renal artery was clamped by non-invasive artery in IR group and P IR group. The left renal artery was clamped by non-invasive artery in IR group and P IR group. After reperfusion, the rats in group S and group IR were fed with probucol daily with warm boiled water every day. All the rats were killed after 1 week. Blood and renal tissue samples were collected, and the blood biochemical indexes of the rats were detected. Renal histopathological changes were observed. Results compared with IR group, serum urea nitrogen (BUN), serum creatinine (SCr), serum lipid level (P 0.05), serum and renal SOD activity and MDA content were significantly decreased in, P IR group (P0.05). The pathological injury of renal tissue was alleviated and the renal index was significantly improved (P0.05). Conclusion Probucol can improve the activity of SOD in blood and renal tissue, improve renal function, decrease the level of blood lipids, alleviate the pathological injury of renal tissue and play a protective role in renal injury.
【作者单位】： 中国人民解放军武汉总医院肾内科;
【基金】：湖北省卫生计生委科研资助项目(No.WJ2015Z042)
【分类号】：R692
，
本文编号：2373814
[Abstract]:Objective to observe the protective effect of Probucol on renal oxidative stress injury in ischemic reperfusion rats and to explore its mechanism. Methods Thirty male SD rats were randomly divided into three groups: sham operation group (S group), ischemia reperfusion group (IR group) and probucol treatment group (P IR group). 10. P IR rats in each group were treated with 500mg/kg daily. Rats in group S and group IR were perfused with the same amount of boiling water every day. 1 week later, the rat model of renal ischemia-reperfusion was established. The right kidney was excised in three groups, and the left renal artery was clamped by non-invasive artery in IR group and P IR group. The left renal artery was clamped by non-invasive artery in IR group and P IR group. After reperfusion, the rats in group S and group IR were fed with probucol daily with warm boiled water every day. All the rats were killed after 1 week. Blood and renal tissue samples were collected, and the blood biochemical indexes of the rats were detected. Renal histopathological changes were observed. Results compared with IR group, serum urea nitrogen (BUN), serum creatinine (SCr), serum lipid level (P 0.05), serum and renal SOD activity and MDA content were significantly decreased in, P IR group (P0.05). The pathological injury of renal tissue was alleviated and the renal index was significantly improved (P0.05). Conclusion Probucol can improve the activity of SOD in blood and renal tissue, improve renal function, decrease the level of blood lipids, alleviate the pathological injury of renal tissue and play a protective role in renal injury.
【作者单位】： 中国人民解放军武汉总医院肾内科;
【基金】：湖北省卫生计生委科研资助项目(No.WJ2015Z042)
【分类号】：R692
，
本文编号：2373814
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