GSTP1表达下调促进膀胱癌细胞T24凋亡

发布时间：2018-12-14 04:31

【摘要】：目的通过慢病毒表达系统,使用shRNA下调人膀胱癌T24细胞GSTP1基因的表达,研究其对T24细胞增殖的影响,并探讨其可能的作用机制。方法通过慢病毒表达系统,建立稳定低表达GSTP1基因的T24细胞株;进行CCK-8实验以及克隆形成实验,检测GSTP1表达下调对T24细胞增殖的影响;对细胞进行Annexin V-FITC/PI染色,使用流式细胞仪,检测GSTP1表达下调对T24细胞凋亡的影响。结果与未处理组、对照组相比,GSTP1干扰组的T24细胞增殖受到明显抑制,差异有统计学意义(P0.01);细胞凋亡检测结果显示,GSTP1表达下调后明显促进T24细胞凋亡,差异有统计学意义(P0.01)。结论 GSTP1表达下调抑制膀胱癌细胞T24增殖,并其机制可能与促进T24细胞凋亡相关。
[Abstract]:Objective to down-regulate the expression of GSTP1 gene in human bladder cancer cell line T24 by using lentivirus expression system and to investigate its effect on the proliferation of T24 cells and its possible mechanism. Methods T24 cell lines with low expression of GSTP1 gene were established by lentivirus expression system, and the effects of down-regulation of GSTP1 expression on the proliferation of T24 cells were detected by CCK-8 assay and clone formation assay. Annexin V-FITC/PI staining and flow cytometry were used to detect the effect of down-regulation of GSTP1 expression on apoptosis of T 24 cells. Results compared with the untreated group and the control group, the proliferation of T24 cells in the GSTP1 interference group was significantly inhibited (P0.01). The results of apoptosis detection showed that the down-regulated expression of GSTP1 significantly promoted the apoptosis of T24 cells (P0.01). Conclusion the down-regulation of GSTP1 expression inhibits the proliferation of bladder cancer cell line T24, and its mechanism may be related to the promotion of T24 cell apoptosis.
【作者单位】：桂林医学院附属医院泌尿外科;赣州市人民医院泌尿外科;
【基金】：国家自然科学基金(编号:81360377、81660425) 广西自然科学基金(编号:2015jj DA40034、2010GXNSFA183013)
【分类号】：R737.14

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