脂氧素对脂多糖诱导的人近曲肾小管上皮细胞氧化应激的影响及机制

发布时间：2018-12-18 05:18

【摘要】：目的:研究脂氧素对人近曲肾小管上皮细胞(HK2细胞)氧化应激的影响并进一步探讨其内在机制。方法:分别用脂氧素A4(LXA_4)和(或)脂多糖(LPS)刺激HK2细胞,观察两者对细胞形态的影响;免疫荧光检测LXA_4和(或)LPS对HK2细胞核因子E2相关因子2(Nrf2)核转位的影响,RT-PCR检测LXA_4和(或)LPS对HK2细胞Nrf2及下游二期酶分子表达的影响。结果:与对照组相比,LPS促使细胞崩解、坏死,而LXA_4保护细胞免受损伤;免疫荧光显示,LPS能明显降低Nrf2蛋白的表达,并促进其向胞浆转位,而LXA_4能增加Nrf2蛋白的表达,并促进其向核转位;RT-PCR显示,LPS能明显降低Nrf2及下游二期酶m RNA的表达,而LXA_4能逆转这种效应。结论:LXA_4可以明显抑制LPS诱导的HK2细胞氧化应激,其作用可能是通过上调Nrf2通路实现。
[Abstract]:Aim: to investigate the effects of lipoxygenin on oxidative stress in human proximal tubular epithelial cells (HK2 cells) and its underlying mechanism. Methods: HK2 cells were stimulated with lipoxygenA4 (LXA_4) and / or lipopolysaccharide (LPS), respectively. The effect of LXA_4 and / or LPS on the nuclear translocation of HK2 nuclear factor E2 related factor 2 (Nrf2) was detected by immunofluorescence, and the effect of LXA_4 and / or LPS on the expression of Nrf2 and secondary enzyme molecules in HK2 cells was detected by RT-PCR. Results: compared with the control group, LPS induced cell disintegration and necrosis, while LXA_4 protected the cells from injury. Immunofluorescence showed that LPS could significantly decrease the expression of Nrf2 protein and promote its translocation to cytoplasm, while LXA_4 could increase the expression of Nrf2 protein and promote its translocation to nucleus. RT-PCR showed that LPS could significantly decrease the expression of Nrf2 and downstream secondary enzyme m RNA, but LXA_4 could reverse this effect. Conclusion: LXA_4 can significantly inhibit oxidative stress induced by LPS in HK2 cells, which may be mediated by up-regulation of Nrf2 pathway.
【作者单位】：南昌大学第二附属医院特需病房;南昌大学第二附属医院病理科;
【基金】：江西省卫生计生委科技计划课题(编号:20155227)
【分类号】：R692.6

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