GPC5异常表达对前列腺癌细胞生物学行为的影响
发布时间：2018-12-20 10:55
【摘要】：目的 研究GPC5异常表达对前列腺癌细胞生物学行为的影响,并对其相关分子机制进行初步探讨。 方法 1.构建GPC5高/低表达质粒,转染前列腺癌细胞PC3及LNCap,通过与对照组比较,观察分析GPC5异常表达对前列腺癌细胞增殖和侵袭的影响。2.通过提高前列腺癌细胞PC3中c-Myc的表达,观察c-Myc过表达对前列腺癌细胞PC3中GPC5表达的影响。3.利用免疫共沉淀技术及质朴分析,寻找前列腺癌细胞中与GPC5相互作用的生长因子/受体配体。 结果 1.上调GPC5表达可显著抑制前列腺癌细胞PC3的增殖和侵袭能力；下调GPC5使前列腺癌细胞LNCap增殖及侵袭能力显著增强。 2.在前列腺癌细胞PC3中,c-Myc的上调伴随着GPC5的下调, 3.免疫共沉淀及蛋白质朴分析显示在前列腺癌细胞PC3中,肝癌衍生生长因子(HDGF)与GPC5相结合而发挥作用。 结论 1.GPC5异常表达可影响前列腺癌细胞PC3及LNCap的细胞生物学行为,证实GPC5在前列腺癌发生发展中发挥重要作用,可能成为治疗前列腺癌的一个潜在的靶点。 2.c-Myc可调节前列腺癌中GPC5的表达量,进而影响前列腺癌的发生发展。 3.前列腺癌细胞膜表面的GPC5可能作为外源性HDGF在细胞膜表面的共受体并介导其进入细胞内发挥作用。
[Abstract]:Objective to investigate the effect of abnormal expression of GPC5 on biological behavior of prostate cancer cells and its molecular mechanism. Method 1. High / low expression plasmids of GPC5 were constructed and transfected into prostate cancer cells PC3 and LNCap,. The effects of abnormal expression of GPC5 on proliferation and invasion of prostate cancer cells were observed and analyzed. By increasing the expression of c-Myc in prostate cancer cell line PC3, the effect of c-Myc overexpression on the expression of GPC5 in prostate cancer cell line PC3 was observed. 3. 3. By means of immunoprecipitation and simple analysis, the growth factor / receptor ligand interacting with GPC5 in prostate cancer cells was found. Result 1. Upregulation of GPC5 expression significantly inhibited the proliferation and invasion of prostate cancer cell line PC3, and down-regulation of GPC5 significantly enhanced the proliferation and invasion of prostate cancer cell line LNCap. 2. In prostate cancer cell line PC3, the up-regulation of c-Myc was accompanied by down-regulation of GPC5. Immunoprecipitation and proteomic analysis showed that hepatoma derived growth factor (HDGF) combined with GPC5 in prostate cancer cell line PC3. Conclusion the abnormal expression of 1.GPC5 may affect the biological behavior of PC3 and LNCap in prostate cancer cells. It is confirmed that GPC5 plays an important role in the development of prostate cancer and may become a potential target for the treatment of prostate cancer. 2.c-Myc can regulate the expression of GPC5 in prostate cancer, and then affect the development of prostate cancer. 3. GPC5 on the surface of prostate cancer cell membrane may act as a co-receptor of exogenous HDGF on the cell membrane and mediate its entry into the cell.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25
本文编号：2387896
[Abstract]:Objective to investigate the effect of abnormal expression of GPC5 on biological behavior of prostate cancer cells and its molecular mechanism. Method 1. High / low expression plasmids of GPC5 were constructed and transfected into prostate cancer cells PC3 and LNCap,. The effects of abnormal expression of GPC5 on proliferation and invasion of prostate cancer cells were observed and analyzed. By increasing the expression of c-Myc in prostate cancer cell line PC3, the effect of c-Myc overexpression on the expression of GPC5 in prostate cancer cell line PC3 was observed. 3. 3. By means of immunoprecipitation and simple analysis, the growth factor / receptor ligand interacting with GPC5 in prostate cancer cells was found. Result 1. Upregulation of GPC5 expression significantly inhibited the proliferation and invasion of prostate cancer cell line PC3, and down-regulation of GPC5 significantly enhanced the proliferation and invasion of prostate cancer cell line LNCap. 2. In prostate cancer cell line PC3, the up-regulation of c-Myc was accompanied by down-regulation of GPC5. Immunoprecipitation and proteomic analysis showed that hepatoma derived growth factor (HDGF) combined with GPC5 in prostate cancer cell line PC3. Conclusion the abnormal expression of 1.GPC5 may affect the biological behavior of PC3 and LNCap in prostate cancer cells. It is confirmed that GPC5 plays an important role in the development of prostate cancer and may become a potential target for the treatment of prostate cancer. 2.c-Myc can regulate the expression of GPC5 in prostate cancer, and then affect the development of prostate cancer. 3. GPC5 on the surface of prostate cancer cell membrane may act as a co-receptor of exogenous HDGF on the cell membrane and mediate its entry into the cell.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25
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相关期刊论文 前1条
1 纪小微;毛建山;;肝癌衍生生长因子研究进展[J];国际消化病杂志;2012年02期
相关硕士学位论文 前1条
1 刘志飞;GPC5在前列腺癌中的表达及意义[D];天津医科大学;2013年
，本文编号：2387896
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