精子线粒体膜电位与男性精液参数和体质量指数的相关性研究

发布时间：2018-12-28 17:27

【摘要】：目的探讨精子线粒体膜电位(MMP)与精子常规参数及体质量指数(BMI)的相关性。方法在本院辅助生殖科行精液分析的男性患者,禁欲3~7 d后,以手淫方式获取精液样本。通过计算机辅助精液分析仪检测精液常规,改良巴氏染色检查精子形态,JC-1染色后经流式细胞仪评估MMP。结果与对照组(56.68%±11.13%)相比,弱精子组MMP(41.24%±9.71%)显著降低。MMP与BMI呈显著负相关(r=-0.25,P0.01),与精子总活力(r=0.63,P0.01)、前向运动力(r=0.64,P0.01)及正常精子形态率(r=0.37,P0.01)呈显著正相关,而与年龄、精液量、精子浓度和精子数量其余参数的相关性均无统计学意义。结论精液中精子MMP是评估精子功能的重要指标,对临床综合分析男性不育症因素具有一定的参考意义。
[Abstract]:Objective to investigate the correlation between sperm mitochondrial membrane potential (MMP) and sperm normal parameters and body mass index (BMI). Methods semen samples were obtained by masturbation after abstinence for 3 days. Semen routine was detected by computer-assisted semen analyzer, sperm morphology was examined by modified pasteurization staining, and MMP. was evaluated by flow cytometry after JC-1 staining. Results compared with the control group (56.68% 卤11.13%), the MMP of weak sperm group (41.24% 卤9.71%) was significantly lower than that of the control group (41.24% 卤9.71%). There was a significant negative correlation between MMP and BMI (r-0.25, P0.01), and a significant correlation with the total sperm motility (0.63%, P0.01). There was a significant positive correlation between forward motility (r = 0.64) and normal sperm morphology (r = 0.37, P 0.01), but there was no significant correlation with age, semen volume, sperm concentration and other parameters of sperm count. Conclusion sperm MMP in semen is an important index to evaluate sperm function, which has certain reference significance for clinical comprehensive analysis of male infertility.
【作者单位】：上海交通大学医学院附属第九人民医院辅助生殖科;
【基金】：上海市卫生和计划生育委员会青年项目(20164Y0137) 国家自然基金项目(81571486)~~
【分类号】：R698.2

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