Survivin和Vimentin在肾透明细胞癌中的表达及临床意义
发布时间：2019-01-20 17:41
【摘要】：目的研究凋亡蛋白抑制因子(Survivin)和波形蛋白(Vimentin)在肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)中的表达，并分析其与ccRCC临床分期、病理分级和侵袭转移等恶性生物学特性的关系及临床意义。 方法收集2011年10月至2013年4月河南科技大学第一附属医院外科手术切的56例肾透明细胞癌组织标本，年龄32～75岁，，平均年龄57岁，其中男性32例，女性24例。临床分期按Robson法：I期23例，II期15例，III～IV期18例；其中发生淋巴结转移17例。组织学分级根据Fuhrman分级系统进行分级：I级19例、II级26例、III～IV级11例；同时选取25例癌旁及13例正常肾组织作为对照组，所有选取病例术前均未进行放、化疗，术后经病理证实。所有标本10％福尔马林液固定，常规石蜡包埋，采用免疫组化SP法检测Survivin和Vimentin在肾透明细胞癌、癌旁及正常肾组织中的表达情况，并分析其与肾透明细胞癌恶性生物学特性的关系及两者的相关性。本实验数据采用SPSS17.0统计软件进行数据分析，以P0.05为差异有统计学意义。 结果Survivin阳性表达为棕黄色颗粒，主要定位于胞质，部分胞核亦可见着色。Survivin在56例肾透明细胞癌标本中表达显著升高，其在肾透明细胞癌中的表达阳性率与癌旁肾组织及正常肾组织相比，差异有显著统计学意义(P0.01)。Vimentin阳性表达为棕黄色颗粒，主要定位于胞质，靠近胞膜。Vimentin在56例肾透明细胞癌标本中表达显著高于癌旁及正常肾组织，其在实验组中的阳性表达率与对照组相比，有显著差异性(P0.01)。随着肿瘤临床分期和病理分级的升高，Survivin和Vimentin的表达逐渐增强，其在肾透明细胞癌的不同临床分级及病理分级中表达有显著差异性(P0.01)，而两者的表达与患者的性别及年龄均无关，差异无统计学意义(P0.05)。Spearman等级相关分析显示Survivin和Vimentin的表达呈显著正相关(r=0.566, P0.01)。 结论1、Survivin和Vimentin在肾透明细胞癌组织中的表达明显高于癌旁及正常肾组织，提示Survivin和Vimentin在肾透明细胞癌的发生发展过程中发挥重要的作用。 2、肾透明细胞癌中Survivin和Vimentin的表达与临床分期、病理分级及有无淋巴结转移有关，而与性别及年龄无关，提示Survivin和Vimentin可能与肾透明细胞癌的浸润和转移密切相关。 3、Survivin和Vimentin在肾透明细胞癌中的表达呈显著正相关，提示两者可能在肾透明细胞癌的发生发展过程中存在协同作用，联合两个因子的检测可为肾透明细胞癌的恶性生物学特性的判断提供重要的信息。
[Abstract]:Objective to study the expression of apoptosis inhibitor (Survivin) and vimentin (Vimentin) in renal clear cell carcinoma (RCC) (clear cell renal cell carcinoma, ccRCC), and to analyze their correlation with ccRCC clinical stage. The relationship between pathological grading and invasion and metastasis of malignant biological characteristics and its clinical significance. Methods from October 2011 to April 2013, 56 specimens of renal clear cell carcinoma were collected from the first affiliated Hospital of Henan University of Science and Technology. The average age was 57 years, including 32 males and 24 females. According to Robson's method, 23 cases had stage I, 15 cases had stage II and 18 cases had stage III~IV, among which 17 cases had lymph node metastasis. According to the Fuhrman classification system, 19 cases were classified as I grade, 26 cases as II grade and 11 cases as III~IV grade. 25 cases of cancer and 13 cases of normal renal tissue were selected as control group. All the selected cases were not treated with chemotherapy before operation and confirmed by pathology after operation. All specimens were fixed in 10% formalin solution and embedded in paraffin. The expression of Survivin and Vimentin in renal clear cell carcinoma (RCC) and normal renal tissues was detected by immunohistochemical SP method. The relationship between it and malignant biological characteristics of renal clear cell carcinoma was analyzed. The data of this experiment were analyzed by SPSS17.0 software, and the difference was statistically significant with P0.05. Results the positive expression of Survivin was brown granules, mainly located in the cytoplasm and stained in some nuclei. The expression of Survivin was significantly increased in 56 cases of renal clear cell carcinoma. The positive rate of P0.01). Vimentin expression in renal clear cell carcinoma was significantly higher than that in adjacent renal tissues and normal renal tissues (P0.01). Vimentin positive expression was brown granules, mainly located in cytoplasm. The expression of Vimentin in 56 cases of renal clear cell carcinoma was significantly higher than that in adjacent and normal renal tissues. The positive expression rate of Vimentin in the experimental group was significantly higher than that in the control group (P0.01). With the increase of clinical stage and pathological grade, the expression of Survivin and Vimentin increased gradually, and there were significant differences in the expression of Survivin and Vimentin in different clinical and pathological grades of renal clear cell carcinoma (P0.01). There was no significant difference between the expression of Survivin and Vimentin (P0.05). Spearman grade correlation analysis showed that there was a significant positive correlation between the expression of Survivin and Vimentin (r = 0.566, P0.01). Conclusion 1the expression of survivin and Vimentin in renal clear cell carcinoma is significantly higher than that in adjacent normal renal tissue, suggesting that Survivin and Vimentin play an important role in the development of renal clear cell carcinoma. 2. The expression of Survivin and Vimentin in renal clear cell carcinoma was related to clinical stage, pathological grade and lymph node metastasis, but not to sex and age. It suggested that Survivin and Vimentin might be closely related to invasion and metastasis of renal clear cell carcinoma. 3The expression of survivin and Vimentin in renal clear cell carcinoma was positively correlated, suggesting that there may be a synergistic effect between survivin and Vimentin in the development of renal clear cell carcinoma. Combined detection of two factors may provide important information for the judgement of malignant biological characteristics of renal clear cell carcinoma.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.11
本文编号：2412241
[Abstract]:Objective to study the expression of apoptosis inhibitor (Survivin) and vimentin (Vimentin) in renal clear cell carcinoma (RCC) (clear cell renal cell carcinoma, ccRCC), and to analyze their correlation with ccRCC clinical stage. The relationship between pathological grading and invasion and metastasis of malignant biological characteristics and its clinical significance. Methods from October 2011 to April 2013, 56 specimens of renal clear cell carcinoma were collected from the first affiliated Hospital of Henan University of Science and Technology. The average age was 57 years, including 32 males and 24 females. According to Robson's method, 23 cases had stage I, 15 cases had stage II and 18 cases had stage III~IV, among which 17 cases had lymph node metastasis. According to the Fuhrman classification system, 19 cases were classified as I grade, 26 cases as II grade and 11 cases as III~IV grade. 25 cases of cancer and 13 cases of normal renal tissue were selected as control group. All the selected cases were not treated with chemotherapy before operation and confirmed by pathology after operation. All specimens were fixed in 10% formalin solution and embedded in paraffin. The expression of Survivin and Vimentin in renal clear cell carcinoma (RCC) and normal renal tissues was detected by immunohistochemical SP method. The relationship between it and malignant biological characteristics of renal clear cell carcinoma was analyzed. The data of this experiment were analyzed by SPSS17.0 software, and the difference was statistically significant with P0.05. Results the positive expression of Survivin was brown granules, mainly located in the cytoplasm and stained in some nuclei. The expression of Survivin was significantly increased in 56 cases of renal clear cell carcinoma. The positive rate of P0.01). Vimentin expression in renal clear cell carcinoma was significantly higher than that in adjacent renal tissues and normal renal tissues (P0.01). Vimentin positive expression was brown granules, mainly located in cytoplasm. The expression of Vimentin in 56 cases of renal clear cell carcinoma was significantly higher than that in adjacent and normal renal tissues. The positive expression rate of Vimentin in the experimental group was significantly higher than that in the control group (P0.01). With the increase of clinical stage and pathological grade, the expression of Survivin and Vimentin increased gradually, and there were significant differences in the expression of Survivin and Vimentin in different clinical and pathological grades of renal clear cell carcinoma (P0.01). There was no significant difference between the expression of Survivin and Vimentin (P0.05). Spearman grade correlation analysis showed that there was a significant positive correlation between the expression of Survivin and Vimentin (r = 0.566, P0.01). Conclusion 1the expression of survivin and Vimentin in renal clear cell carcinoma is significantly higher than that in adjacent normal renal tissue, suggesting that Survivin and Vimentin play an important role in the development of renal clear cell carcinoma. 2. The expression of Survivin and Vimentin in renal clear cell carcinoma was related to clinical stage, pathological grade and lymph node metastasis, but not to sex and age. It suggested that Survivin and Vimentin might be closely related to invasion and metastasis of renal clear cell carcinoma. 3The expression of survivin and Vimentin in renal clear cell carcinoma was positively correlated, suggesting that there may be a synergistic effect between survivin and Vimentin in the development of renal clear cell carcinoma. Combined detection of two factors may provide important information for the judgement of malignant biological characteristics of renal clear cell carcinoma.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.11
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