青蒿素灌胃对顺铂致小鼠急性肾损伤的防治作用观察

发布时间：2019-02-24 09:54

【摘要】：目的观察青蒿素灌胃对顺铂致小鼠急性肾损伤的防治作用。方法将40只小鼠随机分为5组,分别为模型组和青蒿素低、中、高剂量组和对照组,各8只。模型组给予生理盐水灌胃10 d,第5天单次腹腔注射顺铂10 mg/kg;青蒿素低、中、高剂量组每日给予20、40、80 mg/kg青蒿素灌胃10 d,第5天单次腹腔注射顺铂10 mg/kg。对照组给予生理盐水灌胃,连续10 d。测算各组小鼠肾系数;采用脲酶法检测各组小鼠血清尿素氮(BUN)水平,肌氨酸氧化酶法检测肌酐(Cr)水平;采用WST-1法检测各组小鼠肾组织超氧化物歧化酶(SOD)水平,TBA法检测丙二醛(MDA)水平,微板法检测谷胱甘肽(GSH)水平。结果与对照组相比,模型组小鼠肾系数、血清Cr、血清BUN和肾组织MDA升高(P均0.01),肾组织SOD、GSH下降(P均0.01)。与模型组相比,青蒿素中、高剂量组小鼠肾系数、血清Cr、血清BUN和肾组织MDA降低(P均0.01),肾组织SOD、GSH升高(P均0.01);与青蒿素中浓度组相比,青蒿素高剂量组小鼠肾系数、血清Cr、血清BUN和肾组织MDA更低(P均0.01),肾组织SOD、GSH更高(P均0.01)。结论青蒿素灌胃可以改善顺铂致急性肾损伤小鼠肾功能,起到防治小鼠急性肾损伤的作用,且呈一定的剂量-效应关系。这种作用可能是通过青蒿素抑制肾组织氧化应激实现的。
[Abstract]:Objective to observe the preventive and therapeutic effects of artemisinin on acute renal injury induced by cisplatin in mice. Methods Forty mice were randomly divided into 5 groups: model group, artemisinin low, middle, high dose group and control group, each with 8 mice. The model group was given normal saline for 10 days, and cisplatin was injected intraperitoneally for 10 mg/kg; on the 5th day. In the middle and high dose group, the rats were given 20 ~ 400.80 mg/kg / d artemisinin daily for 10 days, and on the 5th day, cisplatin was injected intraperitoneally for 10 mg/kg.. The control group was given normal saline for 10 days. The kidney coefficient of each group was measured, the serum urea nitrogen (BUN) level and creatinine (Cr) level were detected by urease method and creatinine oxidase method respectively. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) (GSH) were detected by WST-1 method, TBA method and microplate method respectively. Results compared with the control group, the renal coefficient, serum Cr, serum BUN and renal MDA in the model group were higher than those in the control group (P0.01), and the SOD,GSH in the renal tissue decreased (P0.01). Compared with the model group, the kidney coefficient, serum Cr, serum BUN and renal tissue MDA in the high dose group were lower than those in the model group (P0.01), and the renal tissue SOD,GSH was increased (P0.01). Compared with the middle concentration of artemisinin group, the renal coefficient, serum Cr, BUN and renal tissue MDA were lower in the high dose group (P0.01), and the SOD,GSH in renal tissue was higher (P0.01). Conclusion Artemisinin could improve the renal function of mice with acute renal injury induced by cisplatin and prevent and treat the acute renal injury in a dose-effect manner. This effect may be achieved by artemisinin inhibiting oxidative stress in renal tissue.
【作者单位】：吉林医药学院;
【基金】：吉林省科技厅自然科学基金项目(20150101131JC) 吉林省教育厅“十二五”科学技术研究项目(吉教合字2015-405) 吉林省卫生厅科技计划基金资助项目(2013ZC015)
【分类号】：R692

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