半乳糖凝集素-7在人肾移植排斥反应中的初步研究

发布时间：2019-03-08 00:09

【摘要】：半乳糖凝集素-7(Galactose lectin,Galectin-7)是一类能够选择性识别糖结构并与其非共价结合的蛋白质，它广泛存在于从低等生物到哺乳动物等各类生物体内，在组织生长调节、细胞间的黏附、细胞凋亡、肿瘤发生及免疫反应等多种方面发挥着重要的生物学功能。本课题旨在研究探讨galectin-7在人肾移植术后免疫排斥反应中的作用、意义及其与移植免疫排斥反应的相互关系，从而为肾移植排斥反应的诊断及治疗提供新思路。通过收集2011年12月至2013年10月在解放军第309医院行同种异体肾移植的患者65例，根据临床生化指标、HE及Masson染色（Banff2005肾移植排斥反应活检诊断标准）将其分为急性排斥组和慢性排斥组，其中急性排斥组34例，男16例，女18例，年龄22-57岁，平均年龄（43±16.3）岁。慢性排斥31例，男17例，女14例，年龄27-54岁，平均年龄（41±17.8）岁。另选取健康供者30例作为健康对照组，男18例，女12例，，年龄29-37岁，平均年龄（34±13.1）岁。应用免疫组织化学染色法和Western Blot法分别检测各组肾组织中galectin-7的表达分布情况以及各组血清中galectin-7表达的差异。经实验得出以下结果，正常组光镜下肾小球及肾小管结构清晰，未见明显异常， galectin-7表达于肾小管管腔处和动脉平滑肌细胞，其他部位未见其表达。急性排斥组光镜下肾小球系膜基质增生，肾小管明显上皮炎，肾间质内可见大量淋巴细胞浸润，肾间质未见纤维化，肾小管未见萎缩，多数病例肾小管管腔处可见galectin-7表达，部分管腔处galectin-7未见表达。慢性排斥组光镜下肾小球毛细血管系膜增生，肾间质及基质纤维化，肾小管萎缩，未见明显肾小管上皮炎，动脉内膜增生，伴少量淋巴细胞浸润，部分病例肾小管上皮细胞可见galectin-7高表达。Western Blot检测结果为：正常对照组灰度值为（0.32±0.15），急性排斥组灰度值为（0.95±0.11），慢性排斥组灰度值为（1.32±0.19），应用SPSS20.0软件进行统计分量资料以均数±标准差（）,数据采用one-way ANOVA处理，各指标间分别作相关分析，急性排斥组高于正常对照组，P0.05，差异具有统计学意义；慢性排斥组高于正常对照组，P0.05，差异具有统计学意义；慢性排斥组高于急性排斥组，P0.05，差异具有统计学意义。本研究得出galectin-7在肾移植术后排斥反应中发挥作用,可作为移植术后排斥反应监测指标之一，并可能成为治疗移植排斥反应的潜在靶点。
[Abstract]:Galactose lectin-7 (Galactose lectin,Galectin-7) is a kind of proteins that can selectively recognize the structure of sugar and bind to it noncovalently. It exists in a wide range of organisms from low-level organisms to mammals and regulates the growth of tissues. Intercellular adhesion, apoptosis, tumorigenesis and immune response play an important role in biology. The purpose of this study is to study the role and significance of galectin-7 in immune rejection after human renal transplantation and its relationship with allograft rejection, so as to provide a new way of thinking for the diagnosis and treatment of renal transplantation rejection. From December 2011 to October 2013, 65 patients underwent renal allograft in the 309th Hospital of the Chinese people's Liberation Army (PLA), according to clinical biochemical indicators, The patients were divided into acute rejection group (n = 34) and chronic rejection group (n = 34) by HE and Masson staining (diagnostic criteria of Banff2005 renal transplantation rejection biopsy). There were 34 patients with acute rejection, 16 males and 18 females, with an average age of (43 卤16.3) years old (22 卤57 years old) with a mean age of (43 卤16.3) years. 31 cases of chronic rejection, 17 males and 14 females, aged 27 to 54 years, mean age (41 卤17.8) years. In addition, 30 healthy donors (18 males and 12 females, with an average age of (34 卤13.1) years) were selected as the control group. Immunohistochemical staining and Western Blot method were used to detect the expression and distribution of galectin-7 in renal tissue and the difference of galectin-7 expression in serum of each group. The results showed that the structure of glomeruli and tubules was clear and no obvious abnormality was observed in the normal group. The expression of galectin-7 was found in the lumen of renal tubules and arterial smooth muscle cells, but no expression was found in other parts of the renal tubular lumen and artery smooth muscle cells. In acute rejection group, Mesangial matrix proliferation, renal tubule dermatitis, a large number of lymphocyte infiltration in renal interstitial, no fibrosis in renal interstitial, no atrophy of renal tubule, and the expression of galectin-7 in tubular cavity were observed in most of the cases under light microscope, and there was no fibrosis in renal interstitial tissue and no atrophy of renal tubule in acute rejection group. There was no expression of galectin-7 in some of the lumen. In the chronic rejection group, Mesangial hyperplasia of glomeruli, fibrosis of renal interstitial and matrix, atrophy of renal tubule, no obvious supratubular dermatitis, arterial intimal hyperplasia and a small amount of lymphocyte infiltration were observed in the chronic rejection group. The high expression of galectin-7 in renal tubular epithelial cells was observed in some cases. The gray value of normal control group was (0.32 卤0.15), that of acute rejection group was (0.95 卤0.11), and that of acute rejection group was (0.95 卤0.11). The gray value of chronic rejection group was (1.32 卤0.19). SPSS20.0 software was used to carry out statistical component data, mean 卤standard deviation (), data were processed by one-way ANOVA, and correlation analysis was made among the indexes. The acute rejection group was higher than the normal control group, P 0.05, the difference was statistically significant. The chronic rejection group was higher than the normal control group (P 0.05), and the chronic rejection group was higher than the acute rejection group (P 0.05). In this study, we concluded that galectin-7 may play an important role in renal allograft rejection, and may be a potential target for the treatment of renal allograft rejection.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R699.2

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