二甲基亚砜通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎

发布时间：2019-03-10 19:05

【摘要】：目的探讨二甲基亚砜(dimethyl sulfoxide,DMSO)对鱼精蛋白(protamine sulfate,PS)诱导的大鼠间质性膀胱炎(interstitial cystitis,IC)治疗作用。方法 SD雌性大鼠48只,按随机数字表法分为正常组、PS灌注组、PS+DMSO组、盐水灌注组(NS组),每组12只大鼠。正常组大鼠未给予任何处理;PS组用0.5 m L,30 mg/m L的PS膀胱内灌注并在膀胱内保留30 min;PS+DMSO组使用50%DMSO溶解PS,按照0.5 m L,30 mg/m L的PS膀胱内灌注并在膀胱内保留30 min;NS组用0.5 m L生理盐水膀胱灌注并保留30 min,各组大鼠每周处理1次,连续处理4周。4周后,HE和甲苯胺蓝染色用于病理学评分和肥大细胞计数;尿动力学和肌条实验被用于评价大鼠膀胱功能和肌肉收缩性改变;Western blot检测SOD2、GSH-Px、IL-6、IL-1B、TNF-α的表达量。结果与PS组相比,PS+DMSO组大鼠膀胱病理学评分[(3.00±0.00)vs(0.83±0.40),P0.05]、肥大细胞计数[(19.66±2.16)vs(6.16±1.16),P0.05]以及炎症因子IL-6、IL-1B和TNF-α表达(P0.05)显著降低,而抗氧化应激酶(SOD2和GSH)显著恢复(P0.05);在膀胱功能学上,与PS组相比,PS+DMSO组大鼠膀胱排尿频率[(24.83±3.06)vs(9.83±1.47),P0.05]显著降低,排尿间隔[(2.42±0.37)vs(6.40±0.57),P0.05]显著延长,肌肉收缩频率[(6.93±0.97)vs(4.94±0.73),P0.05]显著降低。结论 DMSO可能通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎。
[Abstract]:Aim to investigate the therapeutic effect of dimethylsulfoxide (dimethyl sulfoxide,DMSO) on interstitial cystitis (interstitial cystitis,IC) induced by protamine (protamine sulfate,PS) in rats. Methods 48 female SD rats were randomly divided into three groups: normal group (n = 12), PS group (, PS DMSO group) and saline group (group NS), with 12 rats in each group. The rats in the normal group were not treated with any treatment, and in the PS group, 0.5ml, 30 mg/m / L PS was perfused into the bladder and retained in the bladder for 30 min;. In the PS DMSO group, 50%DMSO was used to dissolve PS, and 30 mg/m / L PS was perfused into the bladder and 30 min; was retained in the bladder. The rats in NS group were perfused with 0.5ml saline and preserved for 30 min, once a week for 4 weeks. After 4 weeks, HE and toluidine blue staining were used for pathological score and mast cell count. Urodynamics and muscle strip tests were used to evaluate bladder function and muscle contractility changes in rats.; Western blot was used to detect the expression of SOD2,GSH-Px,IL-6,IL-1B,TNF- 伪. Results compared with PS group, the score of bladder pathology in, PS DMSO group [(3.00 卤0.00) vs (0.83 卤0.40), P0.05] and mast cell count [(19.66 卤2.16) vs (6.16 卤1.16)]. The expression of inflammatory factors IL-6,IL-1B and TNF- 伪 decreased significantly (P0.05), while antioxidant kinase (SOD2 and GSH) recovered significantly (P0.05). In the bladder function, compared with the PS group, the frequency of urination in the, PS DMSO group [(24.83 卤3.06) vs (9.83 卤1.47), P0.05] was significantly lower, and the urination interval [(2.42 卤0.37) vs (6.40 卤0.57)] was significantly lower than that in the control group [(2.42 卤0.37) vs (6.40 卤0.57)]. The frequency of muscle contraction [(6.93 卤0.97) vs (4.94 卤0.73), P0.05] was significantly decreased. Conclusion DMSO may improve cystitis induced by protamine by reducing inflammation and oxidative stress.
【作者单位】：第三军医大学新桥医院泌尿外科;
【基金】：国家自然科学基金面上项目(81170704)~~
【分类号】：R694.3

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