骨髓间充质干细胞经肾动脉途径不同移植次数对大鼠阿霉素肾病的修复作用比较

发布时间：2019-04-26 04:40

【摘要】：目的比较分析骨髓间充质干细胞(BMSC)经肾动脉途径单次或多次移植于阿霉素肾病大鼠后对其肾功能的改善作用。方法以阿霉素经尾静脉输入构建大鼠慢性肾病模型32只,随机分为M0、M1、M2、M3组(4×n,n=8),另选8只为正常对照组(N组)。以2×106个/ml、单次0.5 ml分别移植BMSC细胞0次(M0组)、1次(M1组)、2次(M2组)、3次(M3组),不进行BMSC移植组别每次操作以L-DMEM培养基0.5 ml作为安慰剂对照,移植间隔为1周。移植前及末次移植后1、2周末检测大鼠血尿素氮、血肌酐、24 h尿蛋白、24 h尿微量蛋白,末次移植1周末制作病理片并于激光聚焦显微镜下观察大鼠肾脏病理和BMSC细胞在肾脏内分布情况。结果 M0、M1、M2、M3组在各观察点血尿素氮、血肌酐、24 h尿蛋白总量、24 h尿微量蛋白均明显高于N组(P0.001),末次移植第1周末M2、M3组24 h尿蛋白总量、24 h尿微量蛋白明显低于M1组(P0.05),M2、M3组间差异无统计学意义(P=0.063)。结论 BMSC经肾动脉途径移植2次治疗大鼠阿霉素肾病效果最宜。
[Abstract]:Aim to compare the effects of bone marrow mesenchymal stem cells (BMSC) on renal function after transplantation of bone marrow mesenchymal stem cells (MSCs) through renal artery in rats with adriamycin nephropathy. Methods Thirty-two rats were randomly divided into three groups: M _ 0, M _ 1, M _ 2, M _ 3 (4 脳 n, n = 8) and control group (n = 8). BMSC cells were transplanted 0 times (M _ 0 group), 1 time (M _ 1 group), 2 times (M _ 2 group) and 3 times (M _ 3 group) with 2 脳 10 ~ 6 cells / ml, for 0.5 ml. No BMSC transplantation group was performed with L-DMEM medium 0.5 ml as placebo control, the interval of transplantation was 1 week. Blood urea nitrogen, serum creatinine, 24-hour urine protein and 24-hour urine microprotein were measured before transplantation and 1 and 2 weeks after the last transplantation. At the end of the first week after the last transplantation, pathological slices were made and the pathological changes and distribution of BMSC cells in the kidney of rats were observed under laser focusing microscope. Results the levels of blood urea nitrogen, serum creatinine, 24 h urinary protein and 24 h urine microprotein in M0, M1, M2 and M3 groups were significantly higher than those in N group at the end of the first week of transplantation (P0.001). At the end of the first week after the last transplantation, the total urine protein content in M3 group and M3 group was significantly higher than that in N group (P0.001). 24 h urinary microprotein was significantly lower than that of M1 group (P0.05), while there was no significant difference between M2 and M3 groups (P < 0. 063). Conclusion Transplantation of BMSC through renal artery has the best effect on adriamycin nephropathy in rats.
【作者单位】：昆明医科大学第二附属医院放射治疗科;
【分类号】：R692

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