特发性膜性肾病患者血清及尿液外泌体中环状RNA的表达研究

发布时间：2019-07-08 11:29

【摘要】：目的:应用基因测序的方法分析特发性膜性肾病患者血清及尿液外泌体中环状RNA的差异表达,为进一步探索环状RNA作为特发性膜性肾病诊断生物标志物及其调控机制研究奠定基础。方法:特发性膜性肾病患者(IMN组)及正常健康对照者(NC组)各取10例为实验对象,分别收集其外周静脉血分离出的血清及晨尿,并应用试剂盒及超速离心法提取各组的外泌体,一系列酶解反应获取外泌体中的环状RNA,然后利用基因测序的方法筛选出差异性表达的环状RNA,并对其靶基因进行分析。结果:与健康对照组相比,特发性膜性肾病患者血清外泌体中环状RNA的种类减少,且主要表现为内含子来源,显著差异性表达的有89种,主要也是内含子来源,其中49种表达上调,40种表达下调;而尿液外泌体中环状RNA的种类增多,且主要表现为外显子来源,显著差异性表达的有60种,但主要是内含子来源,其中54种表达上调,6种表达下调。膜性肾病患者血清与尿液外泌体相比,显著差异性表达的环状RNA有59种,且主要是内含子来源,其中32种表达上调,27种表达下调。结论:特发性膜性肾病患者血清及尿液外泌体中环状RNA的表达具有疾病特异性。显著差异和特异性表达的环状RNA,如chr7:100550808|100551062对应的MUC3A基因可作为特发性膜性肾病潜在的生物学标志物,并且可为特发性膜性肾病发病机制的研究提供参考和补充。
文内图片：

图片说明：血清外泌体沉淀图
[Abstract]:Objective: to analyze the differential expression of annular RNA in serum and urine exocrine bodies of patients with idiopathic membranous nephropathy by gene sequencing, and to lay a foundation for further exploring the use of annular RNA as a biomarker for the diagnosis of idiopathic membranous nephropathy and its regulatory mechanism. Methods: 10 patients with idiopathic membranous kidney disease (IMN group) and 10 normal controls (NC group) were selected as experimental subjects. The serum and morning urine isolated from peripheral venous blood were collected, and the exocrine bodies of each group were extracted by kit and ultracentrifugation. A series of enzyme hydrolysis reactions were used to obtain annular RNA, in exocrine. Then the differentially expressed circular RNA, was screened by gene sequencing and its target gene was analyzed. Results: compared with the healthy control group, the type of cyclic RNA in serum exocrine of patients with idiopathic membranous nephropathy decreased, and the main expression was intron source. 89 kinds of significantly differentially expressed, mainly intron source, of which 49 kinds of expression were up-regulated and 40 kinds of expression were down-regulated. However, the types of cyclic RNA in urine exocrine increased, and the main expression was exons. There were 60 kinds of significantly differentially expressed, but mainly intron sources, of which 54 were up-regulated and 6 were down-regulated. Compared with urine exocrine, there were 59 kinds of annular RNA differentially expressed in serum and urine of patients with membranous nephropathy, and they were mainly derived from introns, of which 32 were up-regulated and 27 were down-regulated. Conclusion: the expression of cyclic RNA in serum and urine exocrine bodies of patients with idiopathic membranous nephropathy is disease-specific. The MUC3A gene corresponding to significant difference and specific expression of circular RNA, such as chr7:100550808 / 100551062, can be used as a potential biomarker for idiopathic membranous nephropathy, and can be used as a reference and supplement for the study of the pathogenesis of idiopathic membranous nephropathy.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R692

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