膀胱尿路上皮癌中PTEN表达与其基因甲基化的关系
发布时间：2019-07-20 11:50
【摘要】：目的探讨膀胱尿路上皮癌(BUCC)中第10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)表达与其基因启动子甲基化的关系。方法应用Western blot、甲基化特异性聚合酶链反应(MSP),检测41例BUCC和18例正常膀胱组织中PTEN蛋白的表达水平及其基因启动子甲基化状态,并分析两者的相关性。结果BUCC中PTEN蛋白表达低于正常膀胱组织(P0.05),而BUCC中PTEN基因启动子甲基化率为53.66%(22/41),高于正常膀胱组织的0.00%(0/18)(P0.05);与甲基化阴性组的BUCC组织相比,22例甲基化阳性组的PTEN表达水平降低(P0.05)。结论 BUCC中PTEN蛋白的表达水平下调,这与其基因启动子高甲基化密切相关。
[Abstract]:Objective to investigate the relationship between the expression of homologous lost phosphatase-tensin gene (PTEN) on chromosome 10 and the methylation of its gene promoter in bladder and urinary tract epithelial carcinoma (BUCC). Methods the expression of PTEN protein and its promoter methylation status in 41 cases of BUCC and 18 cases of normal bladder tissue were detected by Western blot, methylated specific polymerase chain reaction (MSP), and the correlation between them was analyzed. Results the expression of PTEN protein in BUCC was lower than that in normal bladder tissue (P 0.05), while the methylating rate of PTEN gene promoter in BUCC was 53.66% (22 鈮，
本文编号：2516707
[Abstract]:Objective to investigate the relationship between the expression of homologous lost phosphatase-tensin gene (PTEN) on chromosome 10 and the methylation of its gene promoter in bladder and urinary tract epithelial carcinoma (BUCC). Methods the expression of PTEN protein and its promoter methylation status in 41 cases of BUCC and 18 cases of normal bladder tissue were detected by Western blot, methylated specific polymerase chain reaction (MSP), and the correlation between them was analyzed. Results the expression of PTEN protein in BUCC was lower than that in normal bladder tissue (P 0.05), while the methylating rate of PTEN gene promoter in BUCC was 53.66% (22 鈮，
本文编号：2516707
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