雄激素受体及共调节因子在雄激素调控勃起信号通路VIP/cAMP中的作用
发布时间：2021-01-08 07:26
目的1.探讨雄激素对阴茎海绵体平滑肌细胞（corpus cavernosum smooth muscle cells,CCSMCs）自噬和凋亡的影响,重点研究其对自噬和凋亡桥梁分子BECN1:Bcl-2的调节作用。2.探讨雄激素浓度和雄激素受体（androgen receptor,AR）活性变化对血管活性肠多肽（vasoactive intestinal polypeptide,VIP）调控勃起作用的影响。3.探讨雄激素浓度变化对AR及共调节因子表达的影响,AR及共调节因子信号通路与VIP/c AMP/PKA信号通路的相互作用,进而明确低雄激素浓度时勃起功能维持的机制。方法1.雄性SD大鼠随机分为6组:正常对照组（A组）、手术去势组（B组）、去势后补充十一酸睾酮组（C组）、单独给予AR拮抗剂氟他胺组（D组）、去势后给予氟他胺组（E组）、去势后给予十一酸睾酮和氟他胺组（F组）。2.结合体内电刺激海绵体神经测定海绵体内压力和离体平滑肌条实验测定海绵体平滑肌的舒缩功能,来评估各组大鼠勃起功能情况。3.通过HE染色、Masson三染色、透射电镜观察各组大鼠阴茎结构的变化。4.采用ELISA方法...
【文章来源】：上海交通大学上海市 211工程院校 985工程院校 教育部直属院校
【文章页数】：142 页
【学位级别】：博士
【部分图文】：
调控海绵体平滑肌松弛的环核苷酸信号通路示意图
图 2 雄激素受体结构示意图Figure 2 Schematic structure of the androgen receptor——摘自：Shahriar Koochekpour.Asian JAndrol. 2010; 12(5)639-657.雄激素对 AR 及其共调节因子有复杂调控作用；VIP/cAMP/PKA 信号与
图 3 前列腺癌细胞中 VIP/cAMP/PKA 通路与 AR 及共调解因子通路的关系Figure 3 Summary of reviewed prostate cancer pathways and their interactions——摘自：Dennis Merkle, Ralf Hoffmann. Cellular Signaling. 2011; 23: 507-515
【参考文献】：
期刊论文
[1]慢性非细菌性前列腺炎大鼠勃起功能的观察[J]. 王先进,沈周俊,钟山,张存明,张敏光. 中华实验外科杂志. 2012 (06)
[2]电刺激阴茎海绵体神经测定大鼠勃起功能的方法探讨[J]. 王先进,沈周俊,张敏光,钟山,张存明,朱照伟. 临床泌尿外科杂志. 2012(03)
[3]血管活性肠多肽调控大鼠阴茎勃起的年龄及雄激素相关性研究[J]. 张存明,沈周俊,张敏光,钟山,王先进,胡明明. 现代泌尿外科杂志. 2012(01)
[4]参与阴茎勃起的外周递质信号通路及其与ED发生的关系[J]. 付卫华,鄢俊安. 重庆医学. 2010(20)
[5]Preliminary study on androgen dependence of calcitonin gene-related peptide in rat penis[J]. Zhou-Jun Shen~1, Shan-Wen Chen~1, Ying-Li Lu~2, Liao-Yuan Li~1, Xie-Lai Zhou~1, Ming-Guang Zhang~1, Zhao-Dian Chen~1 ~1 Department of Urology, First Affiliated Hospital, ~2 Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. Asian Journal of Andrology. 2005(01)
[6]Effect of androgen deprivation on penile ultrastructure[J]. <author>Zhou-Jim SHEN, Xie-Lai ZHOU, Ying-Li LU, Zhao-Dian CHENDepartment of Urology, First Affiliated Hospital, Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou 310003, China. Asian Journal of Andrology. 2003(01)
[7]Relationship between gene expression of nitric oxide synthase and androgens in rat corpus cavernosum[J]. 沈周俊,陈昭典,陆颖理,陈峰,陈智. Chinese Medical Journal. 2000(12)
本文编号：2964186
【文章来源】：上海交通大学上海市 211工程院校 985工程院校 教育部直属院校
【文章页数】：142 页
【学位级别】：博士
【部分图文】：
调控海绵体平滑肌松弛的环核苷酸信号通路示意图
图 2 雄激素受体结构示意图Figure 2 Schematic structure of the androgen receptor——摘自：Shahriar Koochekpour.Asian JAndrol. 2010; 12(5)639-657.雄激素对 AR 及其共调节因子有复杂调控作用；VIP/cAMP/PKA 信号与
图 3 前列腺癌细胞中 VIP/cAMP/PKA 通路与 AR 及共调解因子通路的关系Figure 3 Summary of reviewed prostate cancer pathways and their interactions——摘自：Dennis Merkle, Ralf Hoffmann. Cellular Signaling. 2011; 23: 507-515
【参考文献】：
期刊论文
[1]慢性非细菌性前列腺炎大鼠勃起功能的观察[J]. 王先进,沈周俊,钟山,张存明,张敏光. 中华实验外科杂志. 2012 (06)
[2]电刺激阴茎海绵体神经测定大鼠勃起功能的方法探讨[J]. 王先进,沈周俊,张敏光,钟山,张存明,朱照伟. 临床泌尿外科杂志. 2012(03)
[3]血管活性肠多肽调控大鼠阴茎勃起的年龄及雄激素相关性研究[J]. 张存明,沈周俊,张敏光,钟山,王先进,胡明明. 现代泌尿外科杂志. 2012(01)
[4]参与阴茎勃起的外周递质信号通路及其与ED发生的关系[J]. 付卫华,鄢俊安. 重庆医学. 2010(20)
[5]Preliminary study on androgen dependence of calcitonin gene-related peptide in rat penis[J]. Zhou-Jun Shen~1, Shan-Wen Chen~1, Ying-Li Lu~2, Liao-Yuan Li~1, Xie-Lai Zhou~1, Ming-Guang Zhang~1, Zhao-Dian Chen~1 ~1 Department of Urology, First Affiliated Hospital, ~2 Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. Asian Journal of Andrology. 2005(01)
[6]Effect of androgen deprivation on penile ultrastructure[J]. <author>Zhou-Jim SHEN, Xie-Lai ZHOU, Ying-Li LU, Zhao-Dian CHENDepartment of Urology, First Affiliated Hospital, Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou 310003, China. Asian Journal of Andrology. 2003(01)
[7]Relationship between gene expression of nitric oxide synthase and androgens in rat corpus cavernosum[J]. 沈周俊,陈昭典,陆颖理,陈峰,陈智. Chinese Medical Journal. 2000(12)
本文编号：2964186
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