C促红细胞生成素在脑梗死后微循环再通中的作用

发布时间：2018-04-18 07:27

本文选题：脑梗塞 + 微循环　；参考：《中国组织工程研究》2017年16期

【摘要】：背景:C促红细胞生成素可明显提升脑梗死患者预后,且促进心肌梗死后脑微循环再生。目的:探究C促红细胞生成素提升脑梗死患者预后的具体机制。方法:150只Wistar大鼠,随机取120只建立大鼠脑梗死动物模型后分为:诱导脑梗死组,诱导脑梗死组+C促红细胞生成素处理(浓度500,1 000,2 000 U/kg)组,另30只大鼠为空白对照。体外分离并培养脑梗死动物模型微循环血管内皮细胞,CCK8实验检测细胞增殖情况;Western blot检测与增殖相关基因(Ki67,p16)和血管内皮生长因子蛋白表达,RNA干扰技术选择性沉默血管内皮生长因子蛋白的表达,重复上述指标检测。结果与结论:(1)CCK8实验显示,随着C促红细胞生成素浓度的上升,微循环血管内皮细胞的增殖能力也同步上调;Western blot显示,增殖相关基因(Ki67,p16)和血管内皮生长因子蛋白表达水平也显著上调;(2)shR NA-VEFG干扰后,CCK8及Western blot实验显示,微循环血管内皮细胞的增殖能力及增殖相关基因(Ki67,p16)的表达未随C促红细胞生成素浓度的上调成正比;(3)结果提示,C促红细胞生成素通过上调血管内皮生长因子促进脑梗死动物模型微循环血管内皮细胞的增殖。
[Abstract]:Background: Erythropoietin can significantly improve the prognosis of patients with cerebral infarction and promote cerebral microcirculation regeneration after myocardial infarction.Objective: to explore the mechanism of C-erythropoietin in improving prognosis of patients with cerebral infarction.Methods one hundred and fifty Wistar rats were randomly divided into three groups: the cerebral infarction group, the C erythropoietin treatment group (500 ~ 1 000 U / kg), and the other 30 rats as the blank control.Cell proliferation was detected by CCK8 assay in vitro and isolated and cultured in animal model of cerebral infarction. Western blot was used to detect proliferation-related gene (Ki67) p16) and vascular endothelial growth factor (VEGF) protein expression was selectively silenced by RNA-interference technique.Expression of skin growth factor protein,Repeat the above indicators.Results and conclusion CCK8 assay showed that with the increase of erythropoietin concentration, the proliferative ability of vascular endothelial cells in microcirculation was also up-regulated by Western blot.The expression of proliferation-related gene (Ki67 p16) and vascular endothelial growth factor (VEGF) was also significantly up-regulated by CCK8 and Western blot after interference by 2shR NA-VEFG.The proliferative ability of microcirculation vascular endothelial cells and the expression of proliferation-related gene Ki67 p16) were not directly proportional to the concentration of erythropoietin. The results suggested that Erythropoietin promoted by up-regulation of vascular endothelial growth factor.Proliferation of vascular endothelial cells in cerebral infarction animal model.
【作者单位】：河南大学淮河医院神经内科;
【基金】：河南省教育厅自然科学研究计划项目(2013B206253)~~
【分类号】：R743.33

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