组织工程骨修复骨缺损的研究进展及临床应用

  本文关键词：组织工程骨修复骨缺损的研究进展及临床应用，由笔耕文化传播整理发布。

骨缺损

中国组织工程研究与临床康复 第14卷 第24期 2010–06–11出版

Journal of Clinical Rehabilitative Tissue Engineering Research June 11, 2010 Vol.14, No.24

组织工程骨修复骨缺损的研究进展及临床应用★

陈 欣，张春林

Research progress and clinical application of repairing bone defects using tissue engineered bone

Chen Xin, Zhang Chun-lin

Abstract

Department of Orthopedics, the Sixth People’s

Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China;

Chen Xin★, Studying for master’s degree, Department of Orthopedics, the Sixth People’s

Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China seahorse127@

Correspondence to: Zhang Chun-lin, Associate professor, Associate chief physician, Department of Orthopedics, the Sixth People’s

Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China shzhangchunlin@

Received: 2010-01-16 Accepted: 2010-02-09

BACKGROUND: With the development of biotechnology, repairing bone defects using tissue engineered bone has become a hot spot.

OBJECTIVE: To overview the research progress and clinical applications of repairing bone defects with tissue engineered bone. METHODS: The databases of PubMed, Springer Link, and Science Direct were retrieved for papers published from January 2000 to March 2009 with key words of “tissue engineering of bone, bone defect, and clinical applications”. The relevant papers

concerning stem cell, scaffold material, animal model, vascularization during repairing bone defects with tissue engineered bone were collected.

RESULTS AND CONCLUSION: Bone defect reparation has been a clinical challenge. The traditional autogenous bone

transplantation was the gold standard for local bone defects, which had drawback of complication. It is an ideal technique that applying allogeneic bone scaffolds without number restrictions to repair bone defects, which had possibility of rejection reaction and infection. Therefore, the applications of tissue engineered bone are the tendency. The tissue engineered bone comprised 4 key elements: bone conductive scaffolds, releasing osteogenic growth factor, loaded osteogenic potential cells, as well as vascularization. Thus, the factors involved in osteanagenesis and its relations, as well as animal model studies should be evaluated during bone defects reparation. There have been shown positive roles and considerable development prospects of tissue engineered bone, but many issues have to be solved before the clinical applications.

Chen X, Zhang CL. Research progress and clinical application of repairing bone defects using tissue engineered bone.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2010;14(24):4486-4490. [ ]

摘要

背景：随着生物技术的兴起，以生物材料来制作移植替代物的组织工程学正在不断地发展，因此组织工程骨修复骨缺损也成为当前的研究热点。

目的：概述国际上关于骨组织工程研究的现状及临床应用的进展。

方法：应用计算机检索PubMed数据库、Springer Link数据库、Science Direct数据库2000-01/2009-03文献，检索词为 “tissue engineering of bone，bone defect，clinical applications”。选择与组织工程骨修复骨缺损相关的种子细胞、支架材料、动物模型、血管化和临床应用的文章， 无论观察对象为人或动物均纳入检索标准。

结果与结论：骨缺损的修复是临床上的难题之一, 传统吻合血管的自体骨移植是治疗局部骨缺损的金标准，但是其存在供骨区并发症及数量有限的缺点。而无活性的异体骨虽然不受数量的限制，但是存在排异和传染疾病的可能。因此利用组织工程技术构建组织工程骨是修复骨缺损的趋势。组织工程骨包含具有骨传导性的支架、释放诱导成骨的生长因子、负载具有成骨潜能的细胞及组织工程骨血管化或充足的血供4个关键元素，因此要准确地评估组织工程骨修复骨缺损的作用，需要进一步理解参与骨再生的各种因素及其相互作用，同时还要在标准化动物模型研究中衡量积极因素和可能的有害影响。骨组织工程在实验研究领域已经显示出了积极的作用和相当大的发展前景，但在进入临床应用以前还有很多问题等待解决。 关键词：组织工程骨；骨缺损；临床应用；修复；血管化；种子细胞 doi:10.3969/j.issn.1673-8225.2010.24.027

陈欣，张春林．组织工程骨修复骨缺损的研究进展及临床应用[J].中国组织工程研究与临床康复，2010，14(24):4486-4490. [ ]

上海交通大学附属第六人民医院骨科，上海市 200233

陈 欣★，男，1984年生，浙江省东阳市人，汉族，上海交通大学在读硕士，主要从事骨创伤与骨组织工程的研究。 seahorse127@

通讯作者：张春林，副教授，副主任医师，上海交通大学附属第六人民医院骨科，上海市 200233 shzhangchunlin@

中图分类号:R318 文献标识码:A

文章编号: 1673-8225 (2010)24-04486-05

收稿日期：2010-01-16 修回日期：2010-02-09 (20090930003/GW·Z)

0 背景 由创伤、炎症、肿瘤的外科治疗所导致的骨缺损特别是长段骨缺损，是除了由骨折所致的骨不连外，，引起功能丧失并影响生活质量的主要原因。目前传统自体松质骨移植是治疗局部骨缺损的金标准，但是其存在不可否认的局限性，如供骨区损伤、术后并发症和治疗费用等问题，更重要的是来源与数量的限制。异体

骨移植则不受形状与数量的限制，可达到即时的骨量要求，其骨愈合过程与传统的长段自体移植骨基本类似。但是异体骨经各种灭菌处理，缺乏自身成骨作用，骨愈合过程缓慢且多存在不同程度的排斥反应，因此效果欠佳。随着生物材料学的兴起，以生物材料来制作移植替代物的组织工程学正在不断地发展，因此组织工程骨修复骨缺损也成为当前的研究热点。

组织工程学的基本原理是将种子细胞在体外培养扩增后，接种于预先设计的负载生物

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  本文关键词：组织工程骨修复骨缺损的研究进展及临床应用，由笔耕文化传播整理发布。