软骨细胞-海藻酸钠水凝胶微球修复大鼠颅骨缺损

发布时间：2018-03-16 09:16

本文选题：组织工程　切入点：软骨细胞　出处：《第三军医大学学报》2017年20期 　论文类型：期刊论文

【摘要】：目的探讨软骨细胞-海藻酸钠水凝胶修复骨缺损的可行性,比较其与单纯海藻酸钠水凝胶修复效果的差别。方法取SD大鼠乳鼠膝关节软骨分离培养获得第2代软骨细胞,以1×106/m L接种于海藻酸钠水溶液中培养并交联形成复合水凝胶微球。取12只2月龄雄性SD大鼠,建立颅顶单侧缺损模型,随机分为实验组与对照组,分别植入软骨细胞-海藻酸钠水凝胶和单纯海藻酸钠水凝胶微球。于术后6周取材行大体及组织学观察以及Micro-CT扫描定量分析。结果大体观察示术后6周实验组缺损已基本修复完全,表面光滑无塌陷或碎裂,质地较硬接近新生骨组织;对照组缺损明显,周围为不规则骨和纤维结缔样组织。Micro-CT扫描示实验组颅骨缺损得到良好的修复重建,定量分析示实验组骨矿物质密度、骨组织体积分数及骨小梁数目均高于对照组,差异有统计学意义(P0.05)。组织学观察示术后6周实验组缺损区由新生骨组织修复,含少量软骨及纤维结缔组织,出现血管结构;对照组缺损明显,边缘主要由纤维结缔组织和少量新生骨组织构成。结论海藻酸钠复合软骨细胞制备的水凝胶微球对于大鼠颅骨缺损具有一定的修复效果。
[Abstract]:Objective to investigate the feasibility of repairing bone defect by chondrocyte sodium alginate hydrogel and compare the effect of sodium alginate hydrogel alone. Methods the second generation of chondrocytes were obtained from the articular cartilage of neonatal SD rats. The compound hydrogel microspheres were cultured and crosslinked in sodium alginate solution with 1 脳 10 6 / mL of 1 脳 10 6 mL. Twelve 2-month-old male SD rats were used to establish a unilateral cranial defect model and were randomly divided into two groups: experimental group and control group. Chondrocyte sodium alginate hydrogel and sodium alginate hydrogel microspheres were implanted respectively. Gross and histological observation and quantitative analysis of Micro-CT scanning were performed 6 weeks after operation. The gross observation showed that the experimental group was deficient 6 weeks after operation. The damage has been basically repaired, There was no collapse or fragmentation on the smooth surface, and the texture was hard and close to the new bone tissue, while the defect in the control group was obvious, and the irregular bone and fibrous connective tissue. Micro-CT scanning showed that the skull defect in the experimental group was well repaired and reconstructed. Quantitative analysis showed that the bone mineral density, volume fraction of bone tissue and the number of trabeculae in the experimental group were higher than those in the control group, and the difference was statistically significant (P 0.05). Histological observation showed that the defect area of the experimental group was repaired by new bone tissue at 6 weeks after operation. There was a small amount of cartilage and fibrous connective tissue, and vascular structure appeared in the control group, but the defect was obvious in the control group. Conclusion the hydrogel microspheres prepared by sodium alginate combined with chondrocytes can repair the skull defect in rats.
【作者单位】：第三军医大学西南医院关节外科中心;
【分类号】：R318.08;R68

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