骨形态发生蛋白2聚乳酸缓释微球对兔骨髓间充质干细胞相容性研究

发布时间：2018-04-03 15:03

本文选题：rhBMP-　切入点：PLA　出处：《重庆医学》2015年08期

【摘要】：目的观察重组人骨形态发生蛋白2(rhBMP-2)聚乳酸(PLA)缓释微球对兔骨髓间充质干细胞(BMSCs)相容性。方法通过复乳干燥法制备rhBMP-2-PLA缓释微球,并与兔BMSCs共培养,采用MTT法检测rhBMP-2-PLA缓释微球对细胞的毒性及增殖,并通过倒置显微镜及扫描电镜等评价材料的细胞相容性。结果 rhBMP-2-PLA缓释微球各浓度浸提液与BMSCs培养得出对细胞无毒性。实验组与对照组4个不同时间细胞增殖光密度(OD)值经重复测量方差分析得出时间之间OD值比较,差异有统计学意义(P=0.000);实验组与对照组OD值比较差异有统计学意义(P=0.025),实验组OD值高于对照组,时间与组数有显著交互效应,二者变化趋势明显不同(P=0.006)。倒置显微镜观察材料组细胞增殖正常,扫描电镜发现接种7d后细胞周边有rhBMP-2-PLA缓释微球,细胞生长良好,增殖分裂活跃。结论 rhBMP-2-PLA缓释微球对BMSCs无毒性,具有良好的细胞相容性。
[Abstract]:Objective to observe the compatibility of recombinant human bone morphogenetic protein 2 (rhBMP-2) polylactic acid (PLA) microspheres on rabbit bone marrow mesenchymal stem cells (BMSCs).Methods rhBMP-2-PLA sustained release microspheres were prepared by double emulsion drying method and co-cultured with rabbit BMSCs. The cytotoxicity and proliferation of rhBMP-2-PLA sustained release microspheres were detected by MTT method. The cytocompatibility of the materials was evaluated by inverted microscope and scanning electron microscope.Results the rhBMP-2-PLA sustained-release microspheres were cultured with BMSCs in different concentrations and showed no toxicity to cells.The OD values of the four time groups were compared by repeated analysis of variance.The proliferation of the cells in the material group was normal under inverted microscope. The rhBMP-2-PLA sustained release microspheres were found around the cells 7 days after inoculation. The cells grew well and the proliferation and division were active.Conclusion rhBMP-2-PLA sustained release microspheres are not toxic to BMSCs and have good cytocompatibility.
【作者单位】：遵义医学院第三附属医院骨一科;
【基金】：贵州省省长基金资助项目[2011(25)] 贵州省科学技术基金资助项目(黔科合SY[2010]3101) 贵州省社会公关基金资助项目(遵义市科合社字[2010]015号)
【分类号】：R318.08

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